Rabbit Polyclonal PSMB10 Antibody

中文名： PSMB10抗体
别名： LMP10; MECL1; beta2i

货号: IPDX02882

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/200	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/5000-1/10000	Human,Mouse,Rat

产品详情

Aliases	LMP10; MECL1; beta2i
WB Predicted band size	29 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human PSMB10
Formulation	Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: K562 cell lysate, Primary antibody: P02882(PSMB10 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes
The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P02882(PSMB10 Antibody) at dilution 1/100. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P02882(PSMB10 Antibody) at dilution 1/100. (Original magnification: ×200)

参考文献

以下是关于PSMB10抗体的3篇参考文献(示例格式)：

1. **文献名称**：*The Immunoproteasome Subunit LMP10 (PSMB10) as a Potential Biomarker in Autoimmune Diseases*

**作者**：Smith A, et al.

**摘要**：研究探讨了PSMB10(免疫蛋白酶体亚基)在自身免疫疾病患者中的表达水平，利用特异性抗体检测发现其与疾病活动性相关，提示其可能作为诊断标志物。

2. **文献名称**：*Characterization of a Monoclonal Antibody Targeting PSMB10 in Cancer Proteasome Profiling*

**作者**：Zhang L, et al.

**摘要**：开发了一种靶向PSMB10的单克隆抗体，验证其在肿瘤组织中的特异性结合能力，并证实其在癌症中异常高表达，可能与化疗耐药性相关。

3. **文献名称**：*PSMB10-Deficient Mice Reveal Critical Roles of Immunoproteasomes in Viral Antigen Presentation*

**作者**：Tanaka K, et al.

**摘要**：通过PSMB10基因敲除小鼠模型及抗体标记实验，证明PSMB10缺失导致病毒抗原呈递缺陷，阐明其在适应性免疫中的关键作用。

注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等数据库检索确认。建议使用关键词“PSMB10 antibody”或“proteasome beta 10”结合研究领域(如癌症、免疫)进行精确检索。

背景信息

The PSMB10 antibody targets the proteasome subunit beta 10 (PSMB10), a key component of the immunoproteasome. PSMB10. also known as β2i or LMP10. is a catalytic subunit within the β-ring of the 20S immunoproteasome, a specialized proteasome variant induced by cytokines like interferon-gamma (IFN-γ). Unlike constitutive proteasomes, immunoproteasomes are primarily expressed in immune cells and play a critical role in generating antigenic peptides for major histocompatibility complex (MHC) class I presentation. PSMB10 enhances the cleavage specificity of proteasomes, favoring hydrophobic and basic residues, which optimizes antigen processing for immune surveillance.

PSMB10 antibodies are widely used to study immunoproteasome dynamics in autoimmune diseases, neurodegenerative disorders, and cancer. Dysregulation of PSMB10 has been linked to conditions such as rheumatoid arthritis, Alzheimer’s disease, and tumor immune evasion. Researchers employ these antibodies in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry to assess protein expression, localization, and immunoproteasome activity.

Commercial PSMB10 antibodies are typically raised in rabbits or mice, validated for specificity against recombinant human PSMB10. Studies using these antibodies have elucidated the immunoproteasome's role in inflammation, protein homeostasis, and therapeutic responses, making them essential tools in immunology and biomedical research.