| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PIG54; SCC112; SCC-112 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PDS5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PDS5A抗体的3篇参考文献示例(注：文献信息为模拟，实际引用请核实原文)：
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1. **文献名称**: *PDS5A regulates sister chromatid cohesion and maintains genomic stability*
**作者**: Zhang Y, et al.
**摘要**: 该研究利用PDS5A抗体通过免疫沉淀和Western blot分析，揭示了PDS5A作为cohesin复合体的关键调控因子，在维持姐妹染色单体结合及DNA损伤修复中的作用。实验表明PDS5A缺失导致染色体分离异常。
2. **文献名称**: *Downregulation of PDS5A promotes tumorigenesis in colorectal cancer*
**作者**: Lee S, et al.
**摘要**: 通过PDS5A抗体进行免疫组化分析，研究发现结直肠癌组织中PDS5A表达显著降低，并与其在抑制细胞增殖和迁移中的功能相关，提示其作为潜在抑癌基因的作用。
3. **文献名称**: *Structural insights into PDS5A interaction with cohesin subunits*
**作者**: García-Nieto A, et al.
**摘要**: 结合X射线晶体学和PDS5A抗体的免疫荧光定位，该文献解析了PDS5A与cohesin复合体其他亚基的结合界面，阐明了其维持染色体结构的分子机制。
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建议通过PubMed或Google Scholar以关键词“PDS5A antibody”、“PDS5A cohesin function”进一步筛选实验类文献，重点关注方法学中明确使用该抗体的研究。
The PDS5A antibody is a crucial tool in studying the PDS5A protein, a key component of the cohesin complex involved in chromosome organization and sister chromatid cohesion during cell division. Cohesin, composed of SMC1A, SMC3. RAD21. and regulatory subunits like PDS5A, ensures accurate chromosome segregation, DNA repair, and transcriptional regulation. PDS5A (also known as AFG3L1P or hPDS5) interacts with cohesin to modulate its dynamics, influencing chromatin loop formation and gene expression. Unlike its paralog PDS5B, PDS5A exhibits distinct tissue-specific expression patterns and roles in developmental processes.
Antibodies targeting PDS5A enable researchers to investigate its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These studies are vital for understanding cohesin-related disorders, including Cornelia de Lange syndrome and cancers linked to cohesin mutations. Dysregulation of PDS5A has been implicated in genomic instability and tumorigenesis, making it a potential biomarker or therapeutic target.
Commercial PDS5A antibodies are typically validated for specificity across human and model organisms, though cross-reactivity with PDS5B remains a consideration. Recent research also explores PDS5A's non-canonical roles in DNA replication and stem cell differentiation. Its antibody thus serves as a gateway to unraveling cohesin's multifaceted functions in health and disease.
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