| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RPP29 |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human POP4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于POP4抗体的示例参考文献(内容为虚构示例，仅供参考)：
1. **文献名称**：*Characterization of POP4 Antibody in Human Cell Lines*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种高特异性POP4多克隆抗体，用于检测多种人源细胞系中POP4蛋白的表达。通过Western blot和免疫荧光验证了抗体在细胞核定位中的有效性，并探讨了POP4在RNA加工中的作用。
2. **文献名称**：*POP4 as a Biomarker in Neurodegenerative Diseases*
**作者**：Chen L, et al.
**摘要**：利用POP4单克隆抗体分析阿尔茨海默病患者脑组织样本，发现POP4表达水平显著降低，提示其可能参与tau蛋白调控通路，为疾病机制研究提供了新方向。
3. **文献名称**：*Functional Analysis of POP4 in Ribosome Biogenesis*
**作者**：Yamamoto K, et al.
**摘要**：通过免疫沉淀(IP)结合质谱技术，验证POP4抗体在核仁中的特异性结合，揭示了POP4在核糖体RNA前体加工中的关键作用，为靶向治疗提供潜在靶点。
4. **文献名称**：*Development of a Novel POP4 Antibody for Diagnostic Applications*
**作者**：Rodriguez M, et al.
**摘要**：报道了一种新型高灵敏度POP4抗体，适用于ELISA和免疫组化，成功应用于卵巢癌患者血清样本检测，证明POP4可能作为癌症早期诊断标志物。
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**注**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索。建议使用关键词“POP4 antibody”、“POP4 function”或结合具体研究领域(如“POP4 ribosome”)查找真实文献。
POP4 (Processing of Precursor 4), also known as RPP29. is a protein component of two essential ribonucleoprotein (RNP) complexes: RNase P and RNase MRP. These complexes play critical roles in RNA processing. RNase P is responsible for cleaving the 5' leader sequence of precursor tRNA (pre-tRNA) to generate mature tRNA, while RNase MRP is involved in rRNA maturation (cleaving internal transcribed spacer 1 in rRNA) and mitochondrial DNA replication. POP4 acts as a non-catalytic subunit within these complexes, contributing to their structural stability and enzymatic activity.
POP4 antibodies are valuable tools for studying RNA metabolism, ribosome biogenesis, and cell cycle regulation. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate POP4 expression levels, subcellular localization (primarily nucleolar), and interactions with other RNP components. Dysregulation of POP4 has been implicated in developmental disorders and diseases linked to defective RNA processing, including certain cancers and autoimmune conditions. For example, reduced POP4 expression may impair tRNA/rRNA processing, leading to disrupted protein synthesis and cell proliferation. Its association with cell cycle control also makes POP4 a potential biomarker for proliferative diseases. Antibodies targeting POP4 thus facilitate both basic research on RNA processing mechanisms and clinical studies exploring disease pathogenesis.
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