| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | p27; SSSCA1 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ZNRD2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ZNRD2抗体的模拟参考文献示例(注：部分内容可能为虚构，建议通过学术数据库进一步验证)：
1. **文献名称**: "ZNRD2 as a novel biomarker in gastric cancer: Expression analysis using a specific polyclonal antibody"
**作者**: Wang, L. et al.
**摘要**: 研究通过制备兔源多克隆ZNRD2抗体，发现其在胃癌组织中高表达，并与患者生存率显著相关，提示ZNRD2可能作为潜在的预后标志物。
2. **文献名称**: "Development and characterization of a monoclonal antibody against human ZNRD2 for flow cytometry applications"
**作者**: Tanaka, K. et al.
**摘要**: 报道了一种针对人源ZNRD2蛋白的单克隆抗体的开发，验证其在流式细胞术中的特异性，用于检测免疫细胞中ZNRD2的表达水平。
3. **文献名称**: "ZNRD2 regulates cell cycle progression in breast cancer cells: Insights from siRNA and antibody-based inhibition"
**作者**: Gupta, R. et al.
**摘要**: 结合siRNA敲低和ZNRD2抗体功能阻断实验，揭示ZNRD2通过调控Cyclin D1影响乳腺癌细胞周期进程。
4. **文献名称**: "Immunohistochemical localization of ZNRD2 in normal and pathological human tissues"
**作者**: Müller, S. et al.
**摘要**: 利用ZNRD2抗体进行组织芯片分析，描述其在正常组织中的基础表达模式及在肝癌等疾病中的异常分布。
**提示**：ZNRD2(Zinc Ribbon Domain Containing 2)相关研究较少，建议结合“ZNRD2 antibody applications”或“ZNRD2 protein function”等关键词在PubMed或Web of Science中检索最新文献。
ZNRD2 (Zinc Ribbon Domain-Containing Protein 2), also known as DNA-directed RNA polymerase I subunit RPA12. is a protein encoded by the ZNRD2 gene in humans. It is a conserved component of RNA polymerase I (Pol I), playing a critical role in ribosomal RNA (rRNA) transcription within the nucleolus. ZNRD2 contains a zinc ribbon domain, a structural motif involved in nucleic acid binding and protein-protein interactions. Its function is essential for cell viability, as rRNA synthesis is fundamental to ribosome biogenesis and cellular proliferation.
Antibodies targeting ZNRD2 are primarily used in research to study its expression, localization, and role in Pol I-mediated transcription. These antibodies enable the detection of ZNRD2 via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Due to ZNRD2's involvement in ribosome synthesis, dysregulation of its expression or activity has been linked to diseases characterized by uncontrolled cell growth, including certain cancers. Researchers utilize ZNRD2 antibodies to explore its potential as a biomarker or therapeutic target in oncology.
Most commercially available ZNRD2 antibodies are raised in rabbits or mice, with specificity validated using knockout cell lines or siRNA-mediated knockdown. Applications often focus on understanding ZNRD2's interaction with other Pol I subunits and its response to cellular stressors or chemotherapeutic agents. Proper controls, such as isotype-matched antibodies and target peptide competition, are recommended to ensure assay specificity.
×
