| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SPG33; PROTRUDIN |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ZFYVE27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZFYVE27(Protrudin)抗体的参考文献示例(注：部分内容为示例性概括，非真实文献)：
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1. **文献名称**：*ZFYVE27/Protrudin regulates neurite outgrowth via vesicular trafficking in hippocampal neurons*
**作者**：Matsuda S., Inoue T.
**摘要**：本研究利用ZFYVE27特异性抗体，通过免疫荧光和Western blot技术，揭示了ZFYVE27在神经元内体运输中的作用。实验表明，ZFYVE27敲除导致轴突生长缺陷，提示其通过调控Rab11阳性囊泡运输促进神经突起延伸。
2. **文献名称**：*Loss of ZFYVE27 function in hereditary spastic paraplegia models disrupts endoplasmic reticulum dynamics*
**作者**：Reid E., Crosby A.H.
**摘要**：通过患者来源的细胞系和ZFYVE27抗体检测，研究发现遗传性痉挛性截瘫相关突变导致ZFYVE27蛋白表达异常，内质网与晚期内体膜接触减少，进而影响长链脂肪酸代谢及轴突稳态。
3. **文献名称**：*Characterization of a novel monoclonal antibody against human ZFYVE27 for studying cancer cell invasion*
**作者**：Zhou L., Li W.
**摘要**：本文报道了一种高特异性抗ZFYVE27单克隆抗体的开发，并通过免疫共沉淀和质谱分析验证其靶向性。该抗体在乳腺癌细胞中证实ZFYVE27通过调控MMP分泌促进细胞侵袭。
4. **文献名称**：*Protrudin-mediated membrane trafficking promotes myelination in Schwann cells*
**作者**：Jahn R., Deng F.
**摘要**：利用ZFYVE27抗体进行组织染色，发现其在施万细胞中富集于内体-ERGIC膜结构，并通过与KIF5马达蛋白互导，调控髓鞘形成相关脂质的定向运输，为脱髓鞘疾病研究提供新机制。
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**说明**：上述文献为模拟示例，实际研究需通过学术数据库(如PubMed)检索关键词“ZFYVE27 antibody”“Protrudin antibody”获取真实文献。
The ZFYVE27 antibody is a research tool designed to detect and study the ZFYVE27 protein, also known as Protrudin, encoded by the ZFYVE27 gene. This protein contains a FYVE domain, a zinc finger motif that binds phosphatidylinositol 3-phosphate (PI3P), and plays a role in membrane trafficking, neurite outgrowth, and intracellular vesicle transport. ZFYVE27 is particularly implicated in regulating endoplasmic reticulum (ER)-endosome contact sites, facilitating cargo transfer and maintaining cellular homeostasis. Dysfunction of ZFYVE27 has been linked to hereditary spastic paraplegia (HSP), a neurodegenerative disorder characterized by progressive lower limb spasticity, due to its role in axon elongation and neuronal maintenance.
The ZFYVE27 antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to analyze protein expression, localization, and interactions in various cell types and tissues. It aids in investigating ZFYVE27's involvement in membrane dynamics, autophagy, and neurodegenerative pathways. Researchers also utilize this antibody to explore its interactions with binding partners, such as Rab11 and VAPB, which are critical for ER-endosome tethering. Validated for specificity and sensitivity, the antibody is typically raised in hosts like rabbits or mice, with applications in both basic research and disease model studies. Its development supports ongoing efforts to understand ZFYVE27's mechanistic contributions to neurodegeneration and potential therapeutic targets.
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