| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT17 |
| Uniprot No | Q04695 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-432aa |
| 氨基酸序列 | MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGS TLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLD KVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATV DNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLA RADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLS RIPNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEI SELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVE EQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKK EPVTTRQVRTIVEEVQDGKVISSREQVHQTTR |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于KRT17重组蛋白的参考文献概览：
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1. **文献名称**: *Recombinant KRT17 Protein Promotes Epithelial-Mesenchymal Transition in Cutaneous Squamous Cell Carcinoma*
**作者**: Li X, Wang Y, et al.
**摘要**: 本研究通过表达纯化重组KRT17蛋白，证明其能激活Wnt/β-catenin信号通路，促进皮肤鳞状细胞癌细胞的迁移与侵袭，揭示了KRT17在肿瘤转移中的作用机制。
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2. **文献名称**: *Expression and Functional Characterization of Recombinant Keratin 17 in Wound Healing Models*
**作者**: Chen H, Smith R, et al.
**摘要**: 利用大肠杆菌系统成功表达重组KRT17蛋白，实验显示其可加速小鼠皮肤创伤模型的再上皮化过程，并增强成纤维细胞的增殖活性，提示其在组织修复中的潜在应用。
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3. **文献名称**: *KRT17 as a Biomarker: Purification of Recombinant Protein for Autoantibody Detection in Psoriasis*
**作者**: Gupta S, Patel K, et al.
**摘要**: 通过昆虫细胞表达系统制备高纯度重组KRT17蛋白，证实其在银屑病患者血清中特异性结合自身抗体的能力，为开发新型免疫诊断工具提供了实验依据。
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**备注**：以上文献为模拟示例，实际引用时需根据具体研究检索PubMed、Web of Science等数据库获取真实文献信息。
Keratins are a family of structural proteins that form intermediate filaments in epithelial cells, providing mechanical resilience and regulating cellular processes. Among them, keratin 17 (KRT17) is a type I acidic keratin expressed in basal epithelial layers, hair follicles, and nail beds. It plays roles in tissue repair, cell proliferation, and inflammatory responses. KRT17 is dynamically regulated during wound healing and is overexpressed in pathological conditions such as psoriasis, certain cancers (e.g., breast, pancreatic, and squamous cell carcinomas), and inflammatory skin disorders. Its dysregulation is linked to tumor progression, metastasis, and therapy resistance, making it a potential biomarker and therapeutic target.
Recombinant KRT17 protein is engineered in vitro using expression systems like *E. coli* or mammalian cell lines to produce purified, biologically active forms. This recombinant protein retains functional domains, including the helical rod region critical for filament assembly and the head/tail domains involved in protein interactions. Researchers utilize it to study KRT17's molecular mechanisms, including its role in signaling pathways (e.g., mTOR, EGFR), cell adhesion, and stress responses. It also serves as an antigen for antibody development, a standard in diagnostic assays, and a tool for screening therapeutic agents targeting KRT17-associated diseases. Production typically involves affinity chromatography and quality control steps to ensure purity and structural integrity, enabling reproducible experimental outcomes in both basic and translational research.
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