| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT16 |
| Uniprot No | P08779 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS |
| 预测分子量 | 58.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT16重组蛋白的参考文献示例(内容基于学术研究主题，作者和文献名为虚构示例)：
1. **《Recombinant KRT16 Protein Expression and Its Role in Epidermal Barrier Function》**
- 作者：Smith A, et al.
- 摘要：研究通过大肠杆菌系统成功表达并纯化KRT16重组蛋白，发现其参与调控表皮细胞间黏附，并揭示其在银屑病中异常表达对皮肤屏障功能的影响。
2. **《KRT16 Recombinant Protein Promotes Tumor Cell Migration via EGFR Signaling Pathway》**
- 作者：Chen L, et al.
- 摘要：利用哺乳动物细胞表达KRT16重组蛋白，证实其通过与EGFR相互作用激活下游MAPK通路，促进头颈部鳞状细胞癌的侵袭转移。
3. **《Structural Analysis of Recombinant Human KRT16 Using Cryo-EM》**
- 作者：Wang Y, et al.
- 摘要：通过冷冻电镜解析KRT16重组蛋白的分子结构，发现其C端结构域对中间丝蛋白组装的关键作用，为遗传性掌跖角化症相关突变研究提供依据。
4. **《KRT16 Recombinant Protein Accelerates Wound Healing in Diabetic Mouse Models》**
- 作者：Kim J, et al.
- 摘要：在糖尿病小鼠模型中，局部应用KRT16重组蛋白显著增强角质形成细胞增殖和炎症调控，提示其作为慢性伤口治疗靶点的潜力。
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注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索关键词(如"KRT16 recombinant protein")获取。
Keratins are a diverse family of structural proteins essential for maintaining the integrity of epithelial cells. Among them, keratin 16 (KRT16), a type I acidic keratin, has gained attention for its dynamic roles in cellular stress responses and disease pathology. KRT16 is typically co-expressed with its type II partner keratin 6 (KRT6) in stratified epithelia, including the skin, nails, and mucosal tissues. It is constitutively expressed in specialized regions like the palmoplantar epidermis and becomes rapidly upregulated during wound healing, inflammation, or infection, serving as a marker of "activated" keratinocytes.
Structurally, KRT16 contains the conserved central α-helical rod domain flanked by variable head and tail domains, which facilitate filament assembly and protein interactions. Its overexpression is closely linked to hyperproliferative skin disorders such as psoriasis, pachyonychia congenita, and certain cancers. In cancer biology, KRT16 has been implicated in tumor progression, epithelial-mesenchymal transition, and therapy resistance, though its precise mechanisms remain debated.
Recombinant KRT16 protein is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties, post-translational modifications, and interaction networks. Researchers utilize it to investigate its role in cytoskeletal dynamics, cell signaling, and pathogen defense mechanisms. Therapeutic applications include screening for small-molecule inhibitors in keratinopathies or developing biomarkers for epithelial-derived cancers. Challenges persist in replicating native phosphorylation patterns and managing structural complexity during purification. Current research focuses on elucidating its non-mechanical functions, particularly in immunomodulation and cellular stress adaptation, offering potential avenues for targeted therapies.
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