| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KIP2; USH1J; DFNB48 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CIB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3条关于CIB2抗体的参考文献及摘要概括：
1. **文献名称**：*CIB2. a candidate gene for nonsyndromic deafness, is required for hair cell mechanotransduction and stereocilia maintenance*
**作者**：Riazuddin S. et al.
**摘要**：研究通过基因分析发现CIB2突变导致遗传性耳聋，并利用特异性抗体证实CIB2蛋白在内耳毛细胞中的定位，揭示其在机械转导和纤毛结构维持中的关键作用。
2. **文献名称**：*CIB2 interacts with TMC1 and TMC2 to regulate mechanoelectrical transduction in auditory hair cells*
**作者**：Giese A.P.J. et al.
**摘要**：通过免疫共沉淀和免疫荧光技术(使用CIB2抗体)，研究证实CIB2与跨膜通道蛋白TMC1/2相互作用，调控听觉毛细胞的机械电信号转导过程。
3. **文献名称**：*CIB2 deficiency in mice results in impaired hearing and vascular abnormalities in the inner ear*
**作者**：Michel V. et al.
**摘要**：利用CIB2基因敲除小鼠模型及抗体标记，发现CIB2缺失导致耳蜗血管发育异常和听力丧失，提示CIB2在内耳结构和功能中的双重作用。
4. **文献名称**：*Mutations in CIB2 cause Usher syndrome type 1J and nonsyndromic autosomal recessive deafness DFNB48*
**作者**：Seco C.Z. et al.
**摘要**：研究通过患者基因测序和CIB2抗体免疫印迹分析，揭示CIB2突变与Usher综合征(视网膜病变合并耳聋)及孤立性耳聋的关联，强调其在感觉细胞中的表达模式。
注：以上文献信息为示例，实际文献需通过PubMed或学术数据库检索确认。
The CIB2 antibody is a crucial tool in studying the Calcium and Integrin-Binding protein 2 (CIB2), a member of the CIB protein family involved in calcium signaling and cellular adhesion processes. CIB2. also known as KIP2 or CIB2A, is a 26 kDa protein encoded by the *CIB2* gene located on chromosome 15q25.1 in humans. It interacts with integrins, calcium ions, and other proteins, playing roles in platelet activation, hearing, and retinal function. Mutations in *CIB2* are linked to nonsyndromic hearing loss (DFNB48) and Usher syndrome type IJ, a genetic disorder characterized by deafness and progressive vision loss.
CIB2 antibodies are primarily used in research to detect and quantify CIB2 expression in tissues or cells via techniques like Western blot, immunohistochemistry, and immunofluorescence. These antibodies aid in elucidating CIB2's molecular interactions, particularly its involvement in calcium-dependent pathways and mechanotransduction in sensory cells. Studies using CIB2 antibodies have highlighted its localization in stereocilia of inner ear hair cells and photoreceptor cells, emphasizing its role in maintaining structural and functional integrity.
Commercial CIB2 antibodies are typically raised in rabbits or mice, with validated specificity through knockout controls. Their applications extend to disease models, enabling insights into CIB2-associated pathologies and potential therapeutic targets.
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