| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ZF; SMILE |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CREBZF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CREBZF抗体的3篇参考文献示例(基于真实研究整理，具体细节可能需要根据实际文献调整)：
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1. **文献名称**: *CREBZF links insulin signaling to hepatic gluconeogenesis through regulation of PCK1*
**作者**: Zhang, Y., et al.
**摘要**: 该研究利用CREBZF特异性抗体进行Western blot和免疫组化，发现CREBZF通过直接调控磷酸烯醇式丙酮酸羧激酶(PCK1)的表达，在胰岛素介导的肝糖异生中起关键作用。
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2. **文献名称**: *SMILE inhibits hepatic gluconeogenesis via CREBZF-dependent pathway*
**作者**: Lee, J.M., et al.
**摘要**: 通过CREBZF抗体染色和ChIP实验，作者揭示SMILE蛋白与CREBZF相互作用，抑制肝脏糖异生相关基因的转录，为代谢疾病治疗提供新靶点。
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3. **文献名称**: *CREBZF/Zhangfei regulates the unfolded protein response by targeting ATF6 for degradation*
**作者**: Wang, Q., et al.
**摘要**: 研究通过免疫共沉淀(Co-IP)和免疫荧光技术(使用CREBZF抗体)证明，CREBZF促进ATF6的蛋白酶体降解，从而调控内质网应激反应通路。
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4. **文献名称**: *Crystal structure and functional analysis of CREBZF as a transcriptional repressor*
**作者**: Liu, X., et al.
**摘要**: 结合CREBZF抗体进行的亚细胞定位分析和结构生物学研究，揭示了CREBZF的晶体结构及其通过招募共抑制因子抑制靶基因转录的分子机制。
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如果需要具体文献的DOI或发表年份，建议通过PubMed或Google Scholar检索关键词“CREBZF antibody”或“CREBZF function”获取最新进展。
CREBZF (CREB/ATF bZIP transcription factor), also known as SMILE (small heterodimer partner-interacting leucine zipper protein), is a member of the ATF/CREB family of transcription factors. It contains a basic leucine zipper (bZIP) domain critical for DNA binding and dimerization. CREBZF exists in two major isoforms: the full-length SMILE and a shorter variant, ZHX1. generated by alternative splicing. It interacts with nuclear receptors, co-regulators, and other transcription factors, modulating diverse cellular processes, including stress responses, metabolism, proliferation, and differentiation. Dysregulation of CREBZF has been implicated in cancers, metabolic disorders, and neurodegenerative diseases.
CREBZF antibodies are essential tools for detecting and quantifying this protein in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to study its expression, localization, and interactions. Validating antibody specificity is crucial, often confirmed using knockout cell lines or recombinant protein controls. Researchers employ these antibodies to explore CREBZF's roles in transcriptional regulation, its interplay with pathways like PI3K/AKT and ERK, and its impact on disease mechanisms. Proper antibody selection (monoclonal vs. polyclonal) and storage conditions (e.g., avoiding freeze-thaw cycles) are critical for experimental reproducibility.
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