| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | SRK; STD; TZK; STCD; ZAP-70 |
| WB Predicted band size | 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ZAP70 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ZAP70抗体的3篇代表性文献及其摘要内容：
---
1. **文献名称**：*ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile*
**作者**：Wiestner, A., Rosenwald, A., Barry, T.S., et al.
**摘要**：该研究通过检测慢性淋巴细胞白血病(CLL)患者的ZAP70蛋白表达，发现ZAP70阳性与免疫球蛋白重链(IgV)基因未突变状态显著相关，且与更差的临床预后相关。研究还揭示了ZAP70阳性CLL患者独特的基因表达谱，提示其可作为分子标志物。
---
2. **文献名称**：*ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia*
**作者**：Rassenti, L.Z., Huynh, L., Toy, T.L., et al.
**摘要**：本研究比较了ZAP70表达与IgVH突变状态对CLL患者疾病进展的预测价值。结果显示，ZAP70阳性患者无进展生存期显著缩短，且其预测能力与IgVH突变状态相当，甚至在某些亚组中更具临床实用性。
---
3. **文献名称**：*ZAP-70 is a novel conditional prognostic biomarker in diffuse large B-cell lymphoma*
**作者**：Mansouri, L., Noerenberg, D., Young, E., et al.
**摘要**：该研究探索了ZAP70在弥漫大B细胞淋巴瘤(DLBCL)中的表达及其预后意义。研究发现，ZAP70高表达与特定分子亚型(如非生发中心B细胞型)相关，且在联合其他标志物(如MYC重排)时，可作为生存率下降的独立预测因子。
---
**备注**：以上文献均聚焦于ZAP70在血液肿瘤中的生物学功能及临床价值，涉及预后评估、分子分型及治疗靶点探索。如需具体引用，建议通过PubMed或期刊官网核对原文细节。
The ZAP70 (zeta-chain-associated protein kinase 70) antibody is a critical tool for studying the role of ZAP70. a protein tyrosine kinase essential in T-cell receptor (TCR) signaling. ZAP70 is part of the Syk family of kinases and is primarily expressed in T cells and natural killer (NK) cells. Upon TCR activation, ZAP70 is recruited to phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) on the CD3ζ chain, leading to its activation. This triggers downstream signaling cascades, including calcium flux, MAPK pathways, and NF-κB activation, which are vital for T-cell differentiation, proliferation, and immune response.
Mutations in the ZAP70 gene are linked to severe combined immunodeficiency (SCID), characterized by dysfunctional T cells and recurrent infections. In research, ZAP70 antibodies are used to detect protein expression via techniques like Western blot, flow cytometry, and immunohistochemistry. Clinically, ZAP70 expression serves as a prognostic marker in chronic lymphocytic leukemia (CLL), where its presence correlates with aggressive disease and poorer outcomes.
ZAP70 antibodies are crucial for elucidating immune dysregulation mechanisms and developing targeted therapies. Their specificity and reliability make them indispensable in both basic immunology research and diagnostic applications.
×
