| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | DAI; DLM1; DLM-1; C20orf183 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ZBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZBP1抗体的3篇代表性文献，简要整理如下：
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1. **文献名称**：*ZBP1/DAI Drives RIPK3-Mediated Cell Death Induced by IFNs in the Absence of RIPK1*
**作者**：Kesavardhana, S. et al.
**摘要**：该研究通过Western blot和免疫共沉淀(使用ZBP1抗体)发现，ZBP1在干扰素刺激下可独立于RIPK1激活RIPK3.触发细胞程序性坏死(necroptosis)，揭示了其在抗病毒免疫中的关键作用。
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2. **文献名称**：*Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis*
**作者**：Thapa, R.J. et al.
**摘要**：研究利用ZBP1抗体进行免疫荧光和基因敲除实验，证明流感病毒RNA通过形成Z-DNA结构激活ZBP1.进而驱动RIPK3依赖的细胞坏死，阐明了ZBP1在宿主抗病毒防御中的分子机制。
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3. **文献名称**：*ZBP1 Promotes Adaptive Antitumor Immunity and Checkpoint Immunotherapy Response*
**作者**：Jiao, H. et al.
**摘要**：通过免疫组化(ZBP1抗体标记肿瘤组织)，发现ZBP1在肿瘤微环境中促进T细胞介导的免疫应答，并与PD-1抑制剂疗效相关，为癌症免疫治疗提供了新靶点。
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如需具体实验细节或更多文献，可进一步补充关键词(如特定疾病模型或技术方法)。
**Background of ZBP1 Antibody**
ZBP1 (Z-DNA-binding protein 1), also known as DAI (DNA-dependent activator of IFN-regulatory factors) or DLM-1. is a cytosolic innate immune sensor that recognizes noncanonical nucleic acid structures, such as Z-form DNA or RNA (Z-DNA/Z-RNA). It is interferon-inducible and plays a critical role in antiviral defense, inflammatory responses, and programmed cell death pathways. ZBP1 activates RIPK3 (receptor-interacting protein kinase 3)-dependent necroptosis, apoptosis, or pyroptosis upon sensing pathogen- or damage-associated nucleic acids, particularly during viral infections. Its signaling often involves the formation of a PANoptosome complex, integrating multiple cell death pathways.
ZBP1 antibodies are essential tools for studying its expression, localization, and function in immune regulation and disease. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ZBP1's role in viral infections (e.g., influenza, herpesviruses), cancer immunity, and autoimmune disorders. Research also focuses on ZBP1's contribution to sterile inflammation in conditions like neurodegeneration or tissue injury. Antibodies targeting specific domains (e.g., Zα for nucleic acid binding) help dissect mechanistic interactions with ligands or downstream effectors like RIPK1/RIPK3. As dysregulated ZBP1 signaling is linked to pathological outcomes, its antibody-based detection aids in therapeutic exploration, including targeting necroptosis in cancer or mitigating excessive inflammation.
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