| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIMK; LIMK-1 |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human LIMK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LIMK1(磷酸化位点Thr508/Ser509或相关抗体应用)的参考文献示例，供参考：
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1. **文献名称**: "LIM kinase 1 coordinates microtubule stability and actin polymerization in human endothelial cells"
**作者**: Gorovoy M, et al.
**摘要**: 该研究使用针对LIMK1 Thr508磷酸化位点的抗体，揭示了LIMK1通过调控cofilin磷酸化平衡微管稳定性和肌动蛋白动力学，影响内皮细胞迁移和血管生成。
2. **文献名称**: "Phosphorylation of LIM kinase-1 by protein kinase C-ε is crucial for human endothelial cell migration"
**作者**: Kobayashi M, et al.
**摘要**: 作者通过特异性抗体(包括识别LIMK1 Thr508的抗体)发现，PKC-ε介导的LIMK1磷酸化促进内皮细胞迁移，为动脉粥样硬化治疗提供潜在靶点。
3. **文献名称**: "RhoA/ROCK signaling regulates cofilin phosphorylation through LIMK1 in tumor cell invasion"
**作者**: Yoshioka K, et al.
**摘要**: 研究利用抗LIMK1(Thr508)抗体证明，RhoA/ROCK通路通过激活LIMK1磷酸化抑制cofilin，增强肿瘤细胞侵袭能力，提示其作为转移标志物的潜力。
4. **文献名称**: "Characterization of a phosphospecific antibody to LIMK1 and its use in functional studies"
**作者**: Edwards DC, et al.
**摘要**: 该文献开发并验证了针对LIMK1 Thr508磷酸化的抗体，证实其在细胞和小鼠模型中检测LIMK1活性变化的可靠性，并应用于神经元突触可塑性研究。
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**注**：上述文献为示例性质，实际研究中需根据具体抗体编号(如Abcam、CST等厂商的抗体编号)查询。若需精确文献，建议通过PubMed或Google Scholar以“LIMK1 pThr508 antibody”等关键词检索。
LIMK1 (LIM kinase 1) is a serine/threonine kinase that plays a critical role in regulating actin cytoskeleton dynamics by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This phosphorylation event stabilizes actin filaments, influencing cell motility, morphogenesis, and synaptic plasticity. LIMK1 is activated by upstream kinases such as ROCK (Rho-associated kinase) and PAK1 (p21-activated kinase 1) through phosphorylation at specific residues, including Thr508 within its activation loop. The LIMK1 (Ab-508) antibody is a specific monoclonal or polyclonal antibody designed to detect LIMK1 phosphorylated at Thr508. a key post-translational modification associated with its kinase activation.
This antibody is widely used in research to study LIMK1 signaling pathways in contexts such as cancer metastasis, neuronal development, and diseases linked to cytoskeletal dysregulation, including Alzheimer’s and intellectual disabilities. By enabling the detection of activated LIMK1. it helps elucidate mechanisms underlying cell migration, invasion, and structural remodeling in physiological and pathological conditions. Validation of LIMK1 (Ab-508) typically includes applications like Western blotting, immunofluorescence, and immunohistochemistry, with specificity confirmed using phosphorylation-deficient mutants or kinase inhibition assays. Its utility extends to both basic research and preclinical studies targeting LIMK1 as a therapeutic candidate.
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