Rabbit Polyclonal PYCARD Antibody

中文名： PYCARD抗体
别名： ASC; TMS; TMS1; CARD5; TMS-1

货号: IPDX03663

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/50-1/200	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/5000-1/10000	Human,Mouse,Rat

产品详情

Aliases	ASC; TMS; TMS1; CARD5; TMS-1
WB Predicted band size	22 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Fusion protein of human PYCARD
Formulation	Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: RAW264.7 cell lysate, Primary antibody: P03663(PYCARD Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute
The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P03663(PYCARD Antibody) at dilution 1/60. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P03663(PYCARD Antibody) at dilution 1/60. (Original magnification: ×200)

参考文献

以下是3篇与PYCARD(ASC)抗体相关的文献摘要及作者信息，供参考：

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1. **文献名称**: *The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization*

**作者**: Ippagunta SK et al.

**摘要**: 本研究利用PYCARD/ASC特异性抗体(Western blot和免疫荧光验证)，揭示了ASC通过调控Dock2-Rac信号通路影响T细胞迁移和免疫突触形成，为炎症小体在适应性免疫中的作用提供了新机制。

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2. **文献名称**: *ASC speck formation as a readout of inflammasome activation in human myeloid cells*

**作者**: Sester DP et al.

**摘要**: 文章通过抗PYCARD抗体(克隆号：TMS-1)检测巨噬细胞中ASC斑点的形成，建立了炎症小体激活的标准化检测方法，并验证了其在脓毒症和自身炎症性疾病研究中的应用价值。

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3. **文献名称**: *Loss of ASC promotes lung tumor development in cigarette smoke-exposed mice*

**作者**: Wu X et al.

**摘要**: 研究使用PYCARD抗体(兔多克隆抗体，CST公司)进行免疫组化分析，发现ASC缺失促进烟草烟雾诱导的肺癌发生，表明PYCARD在抑制肺癌中具有抑癌基因功能。

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**补充说明**：

以上文献均聚焦于PYCARD/ASC在炎症、免疫和癌症中的功能研究，并明确提及抗体的实验应用(如Western blot、免疫荧光、免疫组化)。如需具体实验条件或抗体货号，建议进一步查阅原文方法学部分。

背景信息

The PYCARD antibody targets the protein encoded by the PYCARD gene, also known as ASC (Apoptosis-associated speck-like protein containing a CARD). ASC is a critical adaptor molecule in inflammasome signaling, featuring two key domains: an N-terminal pyrin domain (PYD) and a C-terminal caspase activation and recruitment domain (CARD). These domains enable ASC to bridge interactions between inflammasome sensors (e.g., NLRP3. AIM2) and procaspase-1. facilitating inflammasome assembly. This process drives caspase-1 activation, leading to proteolytic maturation of pro-inflammatory cytokines IL-1β and IL-18 and induction of pyroptosis, a lytic cell death pathway.

ASC is implicated in diverse physiological and pathological processes, including infection response, autoinflammatory disorders (e.g., cryopyrin-associated periodic syndromes), cancer, and neurodegenerative diseases. Dysregulated ASC expression or mutations are linked to aberrant inflammation and disease progression.

PYCARD antibodies are essential tools for studying ASC's role in inflammasome activation, localization, and protein interactions. They are widely used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to assess ASC expression, oligomerization (specks), and colocalization with inflammasome components. Research utilizing these antibodies has advanced understanding of inflammatory pathways and therapeutic targeting of inflammasome-related diseases. Since its discovery in the early 2000s, ASC has remained a focal point in immunology and translational research.