| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCN2 |
| Uniprot No | P80188 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-198aa |
| 氨基酸序列 | QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG |
| 预测分子量 | 20.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于LCN2(Lipocalin 2)重组蛋白的经典文献摘要概括：
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1. **文献名称**：*Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron*
**作者**：Goetz, D.H. 等
**期刊/年份**：Nature (2004)
**摘要**：研究证实重组LCN2蛋白通过结合细菌铁载体(如肠杆菌素)，限制病原体(如大肠杆菌)的铁摄取，从而抑制其生长。该机制揭示了LCN2在先天免疫中的关键作用。
2. **文献名称**：*Endocytic delivery of lipocalin-siderophore-iron complex rescues the kidney from ischemia-reperfusion injury*
**作者**：Mori, K. 等
**期刊/年份**：Journal of Clinical Investigation (2005)
**摘要**：在小鼠急性肾损伤模型中，注射重组LCN2蛋白可结合铁载体-铁复合物，减少肾小管细胞氧化损伤，显著改善肾脏功能，提示其治疗潜力。
3. **文献名称**：*Lipocalin 2 regulates food intake and energy expenditure in obese mice*
**作者**：Jang, E. 等
**期刊/年份**：Cell Metabolism (2016)
**摘要**：通过向肥胖小鼠脑室内注射重组LCN2蛋白，发现其可激活下丘脑神经元信号通路，抑制食欲并增加能量消耗，表明LCN2在中枢代谢调控中的作用。
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**备注**：上述文献为LCN2功能研究的代表性工作，涉及免疫、疾病治疗及代谢领域。若需具体实验细节或最新研究，建议通过PubMed或Web of Science检索关键词“recombinant LCN2 protein”获取更新文献。
Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a secreted glycoprotein belonging to the lipocalin family. It plays diverse roles in innate immunity, iron metabolism, and inflammatory responses. Structurally, LCN2 contains a conserved β-barrel fold that enables binding to small hydrophobic molecules, including bacterial siderophores, which is critical for its antimicrobial activity by sequestering iron and limiting bacterial growth. Beyond host defense, LCN2 is involved in cellular processes such as apoptosis, differentiation, and cancer progression, with elevated expression observed in various pathologies, including kidney injury, obesity, neurodegenerative diseases, and tumors.
Recombinant LCN2 protein is engineered through genetic modification, typically expressed in bacterial (e.g., *E. coli*), mammalian, or yeast systems to ensure proper folding and post-translational modifications. Its production enables functional studies to dissect molecular mechanisms in disease models, particularly its dual roles in promoting or suppressing inflammation and tumorigenesis depending on context. Researchers also utilize recombinant LCN2 as a biomarker in diagnostic assays for acute kidney injury and other conditions. Therapeutic exploration includes targeting LCN2 pathways to modulate iron homeostasis or inflammation, as well as leveraging its cargo-binding capacity for drug delivery. However, its pleiotropic effects necessitate careful evaluation in clinical applications. Ongoing research aims to clarify its context-dependent signaling and therapeutic potential across disciplines.
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