| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LBH |
| Uniprot No | Q53QV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-105aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSIYFPI HCPDYLRSAK MTEVMMNTQP MEEIGLSPRK DGLSYQIFPD PSDFDRCCKL KDRLPSIVVE PTEGEVESGE LRWPPEEFLV QEDEQDNCEE TAKENKEQ |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LBH重组蛋白的虚构参考文献示例(仅供学术写作参考，非真实文献)：
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1. **文献名称**：*Cloning and Expression of Recombinant LBH Protein in E. coli*
**作者**：Zhang Y et al.
**摘要**：本研究成功构建了LBH基因的原核表达载体，并利用大肠杆菌系统高效表达可溶性LBH重组蛋白。通过亲和层析纯化获得高纯度蛋白，Western blot验证其抗原性，为后续功能研究奠定基础。
2. **文献名称**：*LBH Recombinant Protein Inhibits Wnt/β-Catenin Signaling in Cancer Cells*
**作者**：Smith JL et al.
**摘要**：通过体外实验证实，纯化的LBH重组蛋白可通过结合关键信号分子抑制Wnt/β-catenin通路，降低结直肠癌细胞增殖和迁移能力，提示其作为肿瘤治疗靶点的潜力。
3. **文献名称**：*Functional Characterization of LBH in Embryonic Development Using Recombinant Protein*
**作者**：Wang R, Li X
**摘要**：利用斑马鱼模型研究LBH重组蛋白对心脏发育的影响，发现其通过调控Tbx2基因表达参与心腔分化，为先天性心脏病机制提供新见解。
4. **文献名称**：*Optimization of LBH Protein Production in Mammalian Expression Systems*
**作者**：Garcia M et al.
**摘要**：比较HEK293和CHO细胞表达LBH重组蛋白的效率，优化糖基化修饰条件，获得具有天然构象的蛋白，适用于结构生物学研究及药物筛选。
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注：以上内容为模拟文献，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
LBH (Limb-Bud and Heart) is a conserved vertebrate protein encoded by the *LBH* gene, first identified through its critical role in embryonic development. It is notably expressed during limb bud formation and heart morphogenesis, where it regulates progenitor cell proliferation, differentiation, and tissue patterning. Structurally, LBH contains a conserved N-terminal domain and intrinsically disordered regions, enabling dynamic interactions with transcriptional regulators like Wwtr1 (TAZ) and components of the Wnt/β-catenin signaling pathway. These interactions position LBH as a versatile modulator of developmental signaling cascades.
Beyond embryogenesis, LBH has been implicated in diverse physiological and pathological processes. It exhibits context-dependent dual roles in cancer, acting as either an oncogene or tumor suppressor depending on tissue type and microenvironment. For instance, LBH overexpression correlates with poor prognosis in triple-negative breast cancer by promoting epithelial-mesenchymal transition, while its downregulation is associated with colorectal cancer progression. Additionally, LBH contributes to postnatal tissue homeostasis, particularly in hair follicle regeneration and adipose tissue metabolism.
Recombinant LBH protein is typically produced using *E. coli* or mammalian expression systems, often fused with tags (e.g., His-tag, GST) for purification and detection. Its applications span molecular interaction studies, functional assays in developmental biology, and screening for therapeutic compounds targeting LBH-associated pathways. Recent research also explores LBH's involvement in congenital disorders, such as heart malformations and limb reduction defects, underscoring its translational relevance. Despite progress, the precise molecular mechanisms underlying LBH's pleiotropic functions remain an active area of investigation, particularly its epigenetic regulatory potential and crosstalk with other signaling networks.
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