| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMP3 |
| Uniprot No | P08962 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 103-203aa |
| 氨基酸序列 | AGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNV |
| 预测分子量 | 15.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMP3重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*"LAMP3 regulates dendritic cell activation and inflammatory response in autoimmune diseases"*
**作者**：Matsuda Y, et al.
**摘要**：研究揭示了LAMP3重组蛋白在树突状细胞中的表达调控机制，发现其通过调节溶酶体功能影响抗原呈递过程，并参与自身免疫疾病中炎症反应的激活，为靶向LAMP3的免疫治疗提供依据。
2. **文献名称**：*"LAMP3 promotes epithelial-mesenchymal transition and metastasis in lung cancer"*
**作者**：Zhang H, et al.
**摘要**：该文献发现LAMP3重组蛋白在肺癌细胞中高表达，通过激活Wnt/β-catenin通路促进上皮间质转化(EMT)和肿瘤转移，提示其可作为肺癌预后标志物及治疗靶点。
3. **文献名称**：*"Recombinant LAMP3 protein enhances anti-tumor immunity by activating dendritic cells"*
**作者**：Kobayashi T, et al.
**摘要**：研究利用重组LAMP3蛋白刺激树突状细胞，显著提升其抗原呈递能力和T细胞激活效应，在动物模型中证实其增强抗肿瘤免疫应答的潜力，为癌症疫苗开发提供新策略。
LAMP3 (Lysosome-associated membrane protein 3), also known as DC-LAMP or CD208. is a type I transmembrane glycoprotein belonging to the LAMP family. Initially identified as a lysosomal marker in dendritic cells (DCs), it is encoded by the LAMP3 gene located on human chromosome 3. Structurally, it features a heavily glycosylated luminal domain, a transmembrane region, and a short cytoplasmic tail. Unlike LAMP1 and LAMP2. which are ubiquitously expressed in lysosomal membranes, LAMP3 expression is tightly regulated and often induced under specific conditions, such as cellular stress, inflammation, or activation signals in immune cells.
Recombinant LAMP3 protein is engineered for research and therapeutic applications, typically produced using mammalian expression systems to ensure proper post-translational modifications, including glycosylation. The protein’s recombinant form often includes tags (e.g., Fc or His tags) to facilitate purification and detection. Studies highlight its role in immune regulation and cancer biology. For instance, LAMP3 is upregulated in tumor-associated DCs and certain cancer cells (e.g., breast, lung, and pancreatic cancers), where it correlates with metastasis and poor prognosis. It has been implicated in autophagy, antigen presentation, and cell-cell communication via exosome secretion.
Research on recombinant LAMP3 aids in understanding its dual roles: as a potential biomarker for cancer progression and as a modulator of immune responses. Its involvement in interferon signaling pathways and interactions with immune checkpoints (e.g., PD-L1) make it a candidate for immunotherapy studies. However, its exact mechanisms in lysosomal adaptation, stress response, and immune evasion remain under investigation, driving demand for high-purity recombinant proteins to support functional assays, antibody development, and diagnostic tools.
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