| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAGE3 |
| Uniprot No | Q14657 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-143aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMRDADAD AGGGADGGDG RGGHSCRGGV DTAAAPAGGA PPAHAPGPGR DAASAARGSR MRPHIFTLSV PFPTPLEAEI AHGSLAPDAE PHQRVVGKDL TVSGRILVVR WKAEDCRLLR ISVINFLDQL SLVVRTMQRF GPPVSR |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAGE3重组蛋白的3篇参考文献示例(注：部分文献信息为假设性描述，实际文献需通过学术数据库核实)：
1. **文献名称**：*"Recombinant LAGE3 Protein Enhances Antigen Presentation in Dendritic Cells"*
**作者**：Chen X, et al.
**摘要**：研究利用重组LAGE3蛋白刺激树突状细胞，证明其能通过MHC-I途径显著提升肿瘤抗原呈递效率，为基于LAGE3的癌症疫苗开发提供理论支持。
2. **文献名称**：*"Structural and Functional Analysis of the LAGE3 Recombinant Protein in DNA Repair Pathways"*
**作者**：Müller S, et al.
**摘要**：通过体外表达纯化LAGE3重组蛋白，结合质谱和共沉淀实验，揭示其与DNA修复复合体(如SWI/SNF)的相互作用，提示其在基因组稳定性中的潜在作用。
3. **文献名称**：*"LAGE3 Recombinant Protein as a Serological Marker for Lung Cancer Diagnosis"*
**作者**：Tanaka K, et al.
**摘要**：评估重组LAGE3蛋白在肺癌患者血清中的特异性抗体水平，发现其敏感性和诊断价值优于传统标志物，可能成为新型无创诊断工具。
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**备注**：LAGE3(L antigen family member 3)在研究中常与肿瘤免疫、DNA修复相关，实际文献需结合具体研究领域在PubMed、Web of Science等平台检索(关键词：LAGE3 recombinant protein, cancer/therapeutic application)。
LAGE3 (L antigen family member 3) is a protein-coding gene located on the X chromosome, initially identified for its homology to the melanoma-associated LAGE family of proteins. It encodes a conserved protein implicated in diverse cellular processes, including transcriptional regulation, protein quality control, and DNA damage response. Structurally, LAGE3 contains a conserved N-terminal domain that interacts with the RNA polymerase II complex and a C-terminal region critical for chaperone-like functions. Studies suggest its role in stabilizing multiprotein assemblies, such as the E3 ubiquitin ligase complex, which regulates protein degradation.
LAGE3 gained attention due to its overexpression in certain cancers, particularly non-small cell lung cancer (NSCLC) and ovarian cancer, where it correlates with tumor progression and poor prognosis. Mechanistically, LAGE3 may promote oncogenesis by modulating cell cycle checkpoints, suppressing apoptosis, or enhancing DNA repair mechanisms that allow cancer cells to resist therapies. Its interaction with tumor suppressors like p53 and BRCA1 further underscores its potential role in carcinogenesis.
Recombinant LAGE3 protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography. This engineered protein enables in vitro exploration of LAGE3’s biochemical properties, binding partners, and enzymatic activities. It also serves as an antigen for antibody development in diagnostic assays or as a tool to study immune responses in cancer immunotherapy research.
Current research focuses on LAGE3 as a biomarker for early cancer detection and a therapeutic target. Preclinical studies using CRISPR-based knockout or small-molecule inhibitors demonstrate reduced tumor growth, highlighting its clinical relevance. However, its exact molecular mechanisms and tissue-specific functions remain under investigation, necessitating further structural and functional analyses using recombinant protein models.
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