| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KXD1 |
| Uniprot No | Q9BQD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-179aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDLPDSA SRVFCGRILS MVNTDDVNAI ILAQKNMLDR FEKTNEMLLN FNNLSSARLQ QMSERFLHHT RTLVEMKRDL DSIFRRIRTL KGKLARQHPE AFSHIPEASF LEEEDEDPIP PSTTTTIATS EQSTGSCDTS PDTVSPSLSP GFEDLSHVQP GSPAINGRSQ TDDEEMTGE |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KXD1重组蛋白的假设性参考文献示例，供参考(注：文献为虚构，实际研究中请核实)：
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1. **文献名称**：*KXD1 Recombinant Protein Facilitates Vesicular Acidification via Interaction with V-ATPase*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了KXD1重组蛋白，证实其通过KxDL结构域与V-ATP酶复合体亚基结合，调控囊泡酸化过程。体外实验表明，KXD1缺失会导致溶酶体pH失衡，提示其在细胞内运输中的关键作用。
2. **文献名称**：*Expression and Functional Characterization of KXD1 in Neurodegenerative Models*
**作者**：Zhang L, et al.
**摘要**：通过哺乳动物细胞系表达KXD1重组蛋白，发现其与tau蛋白存在相互作用。过表达KXD1可减轻神经细胞中异常蛋白聚集，提示其可能通过调节囊泡运输参与阿尔茨海默病的病理调控。
3. **文献名称**：*Structural Analysis of KXD1 Reveals a Role in ER-Golgi Trafficking*
**作者**：Lee JH, et al.
**摘要**：利用昆虫细胞系统制备KXD1重组蛋白，通过X射线晶体学解析其三维结构，发现C端结构域对结合运输相关蛋白GOLPH3至关重要。功能实验表明KXD1是内质网-高尔基体膜泡运输的关键调控因子。
4. **文献名称**：*KXD1 Recombinant Protein Modulates Apoptosis in Cancer Cells*
**作者**：Wang Y, et al.
**摘要**：通过体外重组KXD1蛋白与Bcl-2家族蛋白的互作实验，发现其通过促进线粒体膜通透性改变诱导癌细胞凋亡。研究为KXD1在肿瘤治疗中的潜在应用提供了依据。
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**注意**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“KXD1 recombinant protein”或结合具体研究领域筛选。建议通过数据库验证文献真实性并补充完整引用信息(期刊、卷号、页码等)。
**Background of KXD1 Recombinant Protein**
KXD1 (KxDL motif-containing protein 1) is a member of the KxDL protein family, characterized by conserved lysine (K), any amino acid (x), aspartic acid (D), and leucine (L) motifs. It is implicated in intracellular membrane trafficking and vesicle transport processes, particularly within the endosomal-lysosomal system. KXD1 interacts with regulatory components of the vacuolar ATPase (V-ATPase) complex, a critical player in organelle acidification and protein degradation. This interaction suggests its role in maintaining pH homeostasis and facilitating cargo sorting in cellular compartments.
As a recombinant protein, KXD1 is engineered for high-purity production using expression systems such as *E. coli* or mammalian cell lines. Recombinant KXD1 retains functional domains, including transmembrane regions and the conserved KxDL motifs, enabling studies on its structural and mechanistic roles. Its recombinant form is widely utilized to investigate molecular pathways linked to neurodegenerative diseases, lysosomal storage disorders, and cancer, where dysregulated membrane trafficking is a hallmark.
Research highlights KXD1’s involvement in neuronal development and synaptic function, with knockout models showing defects in brain morphology. Additionally, it may modulate cellular stress responses by influencing autophagy and apoptosis. The protein’s conserved motifs suggest evolutionary significance across eukaryotes, making it a valuable target for comparative studies.
In summary, KXD1 recombinant protein serves as a tool to dissect membrane trafficking mechanisms and their implications in disease, bridging molecular biology with therapeutic exploration.
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