| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT5 |
| Uniprot No | P13647 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-590aa |
| 氨基酸序列 | MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVS LAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGG AGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLL TPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKW TLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDL VEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEI NFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIA NRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEI DNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLL REYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTGSVSS GYGSGSGYGGGLGGGLGGGLGGGLAGGGSGSYYSSSSGGVGLSGGLSVGG SGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS |
| 预测分子量 | 91 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT5重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*High-yield expression and purification of recombinant human KRT5 in Escherichia coli*
**作者**：Smith, A. et al.
**摘要**：本研究开发了一种利用大肠杆菌表达系统高效生产重组人KRT5蛋白的方法，通过优化密码子使用和纯化步骤，获得了高纯度的KRT5.并验证其与天然蛋白的结构相似性，为体外研究提供了可靠工具。
2. **文献名称**：*Functional analysis of KRT5 mutations in epidermolysis bullosa using recombinant protein models*
**作者**：Johnson, R. et al.
**摘要**：通过体外表达携带致病突变的KRT5重组蛋白，研究者揭示了突变对蛋白稳定性和中间丝组装的影响，解释了其在遗传性大疱性表皮松解症中的病理机制。
3. **文献名称**：*Recombinant KRT5 facilitates 3D skin organoid development by enhancing basement membrane organization*
**作者**：Lee, S. et al.
**摘要**：该研究证明重组KRT5蛋白可促进皮肤类器官中基底膜的组装，并通过与层粘连蛋白的相互作用改善细胞极性，为组织工程和再生医学提供了新策略。
4. **文献名称**：*Interaction network mapping of recombinant KRT5 with epidermal adhesion proteins*
**作者**：Zhang, Y. et al.
**摘要**：利用重组KRT5进行蛋白质相互作用筛选，发现其与整合素β4和桥粒蛋白的协同作用，阐明了KRT5在维持上皮细胞粘附及屏障功能中的关键角色。
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**建议**：如需具体文献，可通过PubMed、Google Scholar等平台检索关键词“recombinant KRT5 protein”或结合研究主题(如疾病机制、蛋白工程)筛选近年发表的文章。
**Background of KRT5 Recombinant Protein**
Keratin 5 (KRT5), encoded by the *KRT5* gene, is a type II intermediate filament protein essential for maintaining structural integrity in epithelial cells. It pairs with type I keratins (e.g., KRT14) to form heterodimeric complexes, creating a resilient cytoskeletal network that protects cells against mechanical stress. KRT5 is predominantly expressed in stratified epithelia, including the skin, esophagus, and cornea, where it localizes to the basal layer of keratinocytes, playing a critical role in cell adhesion, proliferation, and differentiation.
Recombinant KRT5 protein is produced using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to express purified, functional keratin for research and therapeutic applications. Its production often involves codon optimization, fusion tags (e.g., His-tag), and chromatographic purification to ensure solubility and stability, as native keratins are prone to aggregation.
Studying recombinant KRT5 has advanced understanding of epithelial biology and diseases. Mutations in *KRT5* are linked to epidermolysis bullosa simplex (EBS), a blistering skin disorder caused by cytoskeletal fragility. Recombinant KRT5 aids in modeling such pathologies, screening potential therapies, and elucidating mechanisms of wound healing or cancer progression. In oncology, aberrant KRT5 expression correlates with tumor invasiveness in certain carcinomas, making it a biomarker or therapeutic target.
Additionally, recombinant KRT5 serves as a substrate for investigating post-translational modifications (e.g., phosphorylation) that regulate cellular stress responses or intermediate filament dynamics. Its application extends to tissue engineering, where keratin-based biomaterials promote regeneration in damaged epithelial tissues.
Overall, KRT5 recombinant protein bridges basic research and translational medicine, offering insights into epithelial disorders and innovative treatment strategies.
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