| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT20 |
| Uniprot No | P35900 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-424aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGEFDFSRRSFHRSLSSSLQAPVVST VGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMA MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQ IEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADL QGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTV NVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVT VNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSS QLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRL LEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEEN I |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT20重组蛋白的3篇参考文献示例(内容基于真实研究领域方向概括，非真实文献)：
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1. **文献名称**: "Expression and Purification of Recombinant Human Keratin 20 (KRT20) in Escherichia coli"
**作者**: Smith A, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效表达KRT20重组蛋白的优化方法，包括密码子优化、诱导条件及纯化策略，最终获得高纯度蛋白用于抗体制备。
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2. **文献名称**: "KRT20 as a Biomarker in Colorectal Cancer: Development of a Recombinant Protein-Based ELISA"
**作者**: Zhang Y, et al.
**摘要**: 研究利用哺乳动物细胞表达系统制备KRT20重组蛋白，开发了特异性ELISA检测方法，验证了其在结直肠癌患者血清中的诊断潜力及临床应用价值。
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3. **文献名称**: "Structural and Functional Analysis of Recombinant KRT20 in Epithelial Cell Differentiation"
**作者**: Lee JH, et al.
**摘要**: 通过杆状病毒-昆虫细胞系统表达KRT20重组蛋白，解析其与钙粘蛋白的相互作用机制，揭示了KRT20在上皮细胞分化和肿瘤转移中的调控作用。
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注：以上文献名为示例概括，实际文献需通过PubMed或Google Scholar等平台检索关键词(如"recombinant KRT20"、"KRT20 protein expression")获取。
Keratin 20 (KRT20), a member of the type I keratin family, is an intermediate filament protein primarily expressed in differentiated epithelial cells. It plays a structural role in maintaining cellular integrity and resilience against mechanical stress. KRT20 is notably abundant in the gastrointestinal tract, urothelium, and Merkel cells, with its expression tightly regulated during cell differentiation. Unlike other keratins, KRT20 exhibits a restricted tissue distribution, making it a valuable biomarker for specific epithelial malignancies. For instance, it is a well-established marker for colorectal adenocarcinoma and is frequently used in diagnostic pathology to identify metastatic tumors of gastrointestinal origin.
Recombinant KRT20 protein is engineered using biotechnological methods, typically through the expression of cloned human KRT20 genes in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cell lines). This recombinant form retains the immunogenic and functional properties of native KRT20 while ensuring high purity and consistency for research and clinical applications. Its production involves advanced purification techniques like affinity chromatography, followed by rigorous quality control to verify molecular weight (~48 kDa), structural integrity, and absence of contaminants.
In research, recombinant KRT20 serves as a critical tool for studying epithelial cell differentiation, cancer progression, and metastasis mechanisms. It is widely used to generate antibodies for immunohistochemistry (IHC) and immunoassays (ELISA, Western blot), aiding in cancer diagnosis and subclassification. Clinically, KRT20 expression patterns help distinguish between primary and metastatic cancers, assess tumor aggressiveness, and monitor treatment responses. Its role in liquid biopsies as a circulating tumor marker is also under exploration. By enabling standardized and reproducible studies, recombinant KRT20 advances both basic science and translational oncology.
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