| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAP3 |
| Uniprot No | P28838-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMFLLPLPAAGRVVVRRLAVRRFGSRSLSTA DMTKGLVLGIYSKEKEDDVPQFTSAGENFDKLLAGKLRETLNISGPPLKA GKTRTFYGLHQDFPSVVLVGLGKKAAGIDEQENWHEGKENIRAAVAAGCR QIQDLELSSVEVDPCGDAQAAAEGAVLGLYEYDDLKQKKKMAVSAKLYGS GDQEAWQKGVLFASGQNLARQLMETPANEMTPTRFAEIIEKNLKSASSKT EVHIRPKSWIEEQAMGSFLSVAKGSDEPPVFLEIHYKGSPNANEPPLVFV GKGITFDSGGISIKASANMDLMRADMGGAATICSAIVSAAKLNLPINIIG LAPLCENMPSGKANKPGDVVRAKNGKTIQVDNTDAEGRLILADALCYAHT FNPKVILNAATLTGAMDVALGSGATGVFTNSSWLWNKLFEASIETGDRVW RMPLFEHYTRQVVDCQLADVNNIGKYRSAGACTAAAFLKEFVTHPKWAHL DIAGVMTNKDEVPYLRKGMTGRPTRTLIEFLLRFSQDNA |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAP3重组蛋白的3篇参考文献示例(注：内容为模拟概括，具体文献需根据实际检索结果调整)：
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1. **文献名称**：Expression and Characterization of Recombinant Human LAP3 in E. coli
**作者**：Chen X, et al.
**摘要**：研究报道了在大肠杆菌中高效表达人源LAP3重组蛋白的优化策略，通过亲和层析纯化获得高纯度蛋白，并证实其具有亮氨酸氨基肽酶活性，为后续功能研究提供材料基础。
2. **文献名称**：Structural Insights into LAP3 Catalytic Mechanism by Cryo-EM
**作者**：Smith JL, et al.
**摘要**：利用冷冻电镜解析了重组LAP3的三维结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了其催化水解肽键的分子机制。
3. **文献名称**：LAP3 Recombinant Protein Attenuates Oxidative Stress in Neuronal Cells
**作者**：Wang Y, et al.
**摘要**：研究发现重组LAP3蛋白可通过降解氧化损伤相关多肽，减少神经元细胞凋亡，提示其在神经退行性疾病中的潜在治疗价值。
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建议通过PubMed、Web of Science等平台以“LAP3 recombinant protein”“leucyl aminopeptidase 3 expression”为关键词检索最新文献。
Here’s a concise overview of LAP3 recombinant protein:
LAP3 (Leucine Aminopeptidase 3), also known as Cytosol Aminopeptidase or PSA, is a metalloprotease enzyme encoded by the *LAP3* gene in humans. It belongs to the M1 family of aminopeptidases and plays a critical role in protein turnover by catalyzing the cleavage of N-terminal leucine residues from peptides and polypeptides. This enzyme is ubiquitously expressed in various tissues and is primarily localized in the cytoplasm, where it participates in regulating cellular processes such as protein maturation, peptide hormone activation, and antigen presentation.
The recombinant LAP3 protein is engineered through molecular cloning techniques, where the *LAP3* gene is inserted into expression systems (e.g., *E. coli* or mammalian cells) for large-scale production. Purification typically involves affinity chromatography tags (e.g., His-tag) followed by rigorous quality validation. Recombinant LAP3 retains the enzymatic activity of its native counterpart, making it valuable for biochemical studies, drug discovery, and diagnostic applications.
Research has linked LAP3 dysregulation to pathological conditions, including cancer progression, neurodegenerative disorders (e.g., Alzheimer's disease), and immune dysfunction. Its recombinant form enables detailed structural analysis, inhibitor screening, and mechanistic studies of peptide metabolism. Recent studies also explore its potential as a biomarker for certain cancers and a therapeutic target for modulating proteolytic pathways. The protein's zinc-dependent catalytic mechanism and substrate specificity remain active areas of investigation.
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