| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMTOR2 |
| Uniprot No | Q9Y2Q5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMLRPKALTQVLSQANTGGVQSTLLLN NEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDC MEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQVAAS |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Architecture of the Ragulator complex provides insights into the molecular basis of mTORC1 signaling"**
*作者：Yonehara et al.*
摘要：研究解析了Ragulator复合体(含LAMTOR2)的晶体结构，揭示其通过支架作用调控mTORC1信号通路的分子机制，重组蛋白表达用于结构分析。
2. **"LAMTOR2 modulates cadmium-induced autophagy and apoptosis through ROS-mediated regulation of the AKT/mTOR pathway"**
*作者：Chen et al.*
摘要：利用重组LAMTOR2蛋白探究其在镉暴露下通过氧化应激调控自噬与凋亡的机制，证实其通过AKT/mTOR通路影响细胞应激反应。
3. **"The Ragulator complex coordinates lysosomal membrane organization with nutrient sensing"**
*作者：Shen et al.*
摘要：通过重组蛋白实验验证LAMTOR2在Ragulator复合体中的核心作用，阐明其连接溶酶体膜结构与营养信号传导的功能，影响细胞代谢稳态。
4. **"Structural and functional characterization of LAMTOR2 in cancer cell proliferation"**
*作者：Zhang et al.*
摘要：研究重组LAMTOR2蛋白在肿瘤细胞中的功能，发现其缺失抑制mTORC1活性并减缓细胞增殖，为靶向治疗提供潜在策略。
LAMTOR2. also known as p14 or MAPBPIP, is a critical component of the LAMTOR (Late Endosomal/Lysosomal Adaptor and MAPK and mTOR Activator) complex, which plays a central role in regulating mTORC1 (mechanistic target of rapamycin complex 1) signaling. This signaling pathway is essential for cellular responses to nutrient availability, energy status, and stress, influencing processes like cell growth, autophagy, and metabolism. The LAMTOR complex, localized to lysosomal membranes, acts as a scaffold to recruit mTORC1 and its upstream regulators, enabling activation in response to amino acid signals.
Recombinant LAMTOR2 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) to study its structural and functional properties. The protein consists of a conserved N-terminal domain that mediates interactions with LAMTOR1 (p18) and LAMTOR3 (MP1), forming the core of the heterotrimeric LAMTOR complex. This assembly is critical for anchoring the complex to lysosomal membranes and facilitating signal transduction. Recombinant versions often include affinity tags (e.g., His-tag) for purification and detection, enabling in vitro studies on protein-protein interactions, complex assembly, and mTOR pathway modulation.
Research using recombinant LAMTOR2 has advanced understanding of lysosome-mediated nutrient sensing. Dysregulation of LAMTOR2 is linked to diseases such as cancer, neurodegeneration, and immune disorders, making it a potential therapeutic target. Its recombinant form is widely used in biochemical assays, structural studies (e.g., crystallography), and drug screening to identify modulators of mTOR signaling. Additionally, it serves as a tool to investigate autophagy defects or metabolic imbalances associated with LAMTOR complex dysfunction.
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