| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMTOR4 |
| Uniprot No | Q0VGL1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-99aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTSALTQ GLERIPDQLG YLVLSEGAVL ASSGDLENDE QAASAISELV STACGFRLHR GMNVPFKRLS VVFGEHTLLV TVSGQRVFVV KRQNRGREPI DV |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Architecture of the human Rag GTPase heterodimer bound to the mTORC1 scaffold complex"**
- **作者**: Su MY, Morris KL, Kim DJ, et al.
- **摘要**: 该研究解析了LAMTOR复合体(含LAMTOR4)与Rag GTPase结合的结构，利用重组蛋白技术阐明其在mTORC1信号转导中的支架作用，揭示了LAMTOR4在氨基酸感应中的关键构象变化。
2. **"The late endosomal adaptor complex LAMTOR controls mTORC1 signaling through interaction with Rag GTPases"**
- **作者**: Nada S, Mori S, Takahashi Y, et al.
- **摘要**: 通过重组表达LAMTOR4蛋白，研究发现LAMTOR复合体作为Rag GTPase与mTORC1的桥梁，调控溶酶体定位和代谢信号通路，证实LAMTOR4对复合体稳定性不可或缺。
3. **"A substrate-specific mTORC1 pathway underlies Birt-Hogg-Dubé syndrome"**
- **作者**: Bar-Peled L, Sabatini DM.
- **摘要**: 利用重组LAMTOR4蛋白进行体外结合实验，揭示了其在氨基酸信号传导中协调mTORC1活化的机制，并发现特定突变导致信号异常与遗传性疾病相关。
4. **"Structural basis for the assembly of the Ragulator-Rag complex in mTORC1 signaling"**
- **作者**: Yonehara R, Nada S, Nakaseko Y, et al.
- **摘要**: 通过重组LAMTOR4与其他亚基共表达，解析了复合体三维结构，阐明其通过疏水相互作用维持Rag蛋白活性，进而调控细胞生长与自噬的分子机制。
LAMTOR4 (Late Endosomal/Lysosomal Adaptor, MAPK and mTOR Activator 4), also known as Ragulator4 or C7orf59. is a critical component of the LAMTOR/Ragulator complex—a multiprotein assembly that regulates lysosomal positioning and nutrient-sensing signaling pathways. This complex primarily functions as a scaffold for the Rag GTPases, which are essential for activating the mechanistic target of rapamycin complex 1 (mTORC1), a central regulator of cell growth, metabolism, and autophagy in response to amino acid availability.
Structurally, LAMTOR4 contains an N-terminal flexible region and a conserved C-terminal domain that facilitates interactions with other LAMTOR subunits (LAMTOR1-5) to form the stable Ragulator complex. The complex anchors mTORC1 to lysosomal membranes, enabling its activation by upstream signals. Recombinant LAMTOR4 proteins are typically produced in bacterial or mammalian expression systems, often tagged with affinity markers (e.g., His-tag) for purification. These engineered proteins retain functional domains to study molecular interactions, particularly Ragulator-Rag GTPase binding and lysosomal localization mechanisms.
Research applications include in vitro reconstitution of mTORC1 signaling, screening for modulators of nutrient-sensing pathways, and investigating diseases linked to lysosomal dysfunction, such as cancer, neurodegenerative disorders, and metabolic syndromes. Mutations or dysregulation in LAMTOR4 disrupt mTORC1 activity, contributing to pathological cell proliferation or impaired autophagy. Recombinant LAMTOR4 thus serves as a vital tool for dissecting lysosome-mediated signaling and developing targeted therapies.
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