| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CPT1, IIAE4, CPTASE |
| WB Predicted band size | 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CPT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CPT2抗体相关的模拟参考文献示例(注：以下内容为学术场景模拟，实际文献请通过数据库核实)：
1. **文献名称**: "Carnitine palmitoyltransferase II deficiency: Molecular and biochemical analysis using novel monoclonal antibodies"
**作者**: Smith J, et al.
**摘要**: 本研究开发了针对CPT2蛋白的特异性单克隆抗体，用于分析患者成纤维细胞中CPT2的表达水平。通过Western blot和免疫荧光验证抗体特异性，发现CPT2缺陷患者的蛋白表达显著降低，为快速诊断该遗传性脂肪酸氧化障碍提供了可靠工具。
2. **文献名称**: "Immunohistochemical localization of CPT2 in human skeletal muscle and liver tissues"
**作者**: Tanaka K, et al.
**摘要**: 利用抗CPT2多克隆抗体对正常与病理组织进行免疫组化分析，证实CPT2在线粒体内膜的高表达。研究揭示了肌肉组织中CPT2分布异常与运动性横纹肌溶解症的相关性，为病理机制研究提供形态学依据。
3. **文献名称**: "Development of a high-sensitivity ELISA assay for quantifying CPT2 levels in dried blood spots"
**作者**: Garcia R, et al.
**摘要**: 基于抗CPT2抗体的夹心ELISA检测法被开发用于新生儿筛查。该方法在2000份干血片中验证显示，能准确区分健康个体与CPT2突变携带者，灵敏度达98%，显著优于传统生化检测。
4. **文献名称**: "Functional characterization of CPT2 mutations using antibody-based protein stability assays"
**作者**: Müller S, et al.
**摘要**: 通过抗CPT2抗体进行热稳定性实验，发现p.Ser113Leu突变导致蛋白降解加速。研究结合质谱与抗体共沉淀技术，阐明了突变影响酶活性的结构基础，为个性化治疗提供分子依据。
注：实际研究中建议通过PubMed/Google Scholar检索真实文献(可尝试关键词：CPT2 antibody, diagnostic application, immunohistochemistry)，并优先选择近5年高被引论文。
**Background of CPT2 Antibodies**
Carnitine palmitoyltransferase 2 (CPT2) is a mitochondrial enzyme critical for fatty acid β-oxidation, a process that converts fatty acids into energy. Located on the inner mitochondrial membrane, CPT2 facilitates the transfer of long-chain fatty acids from carnitine to coenzyme A, enabling their entry into the mitochondrial matrix for breakdown. Deficiencies in CPT2 function are linked to metabolic disorders, such as CPT2 deficiency, a rare autosomal recessive condition causing muscle weakness, hepatomegaly, and life-threatening complications like hypoglycemia or cardiac arrest during metabolic stress.
CPT2 antibodies are essential tools in research and diagnostics, used to detect and quantify CPT2 protein expression in tissues or cells. These antibodies aid in studying CPT2's role in metabolic regulation, its interaction with other enzymes, and its dysregulation in diseases like obesity, diabetes, and cancer. In diagnostics, they help identify CPT2 deficiency via immunohistochemistry or Western blotting of patient samples.
Commercially available CPT2 antibodies are typically raised in rabbits or mice, targeting specific epitopes of human or murine CPT2. Validation includes testing for specificity (e.g., knockout cell lines) and cross-reactivity. Researchers also utilize these antibodies to explore therapeutic strategies, such as modulating fatty acid metabolism in metabolic syndromes. Overall, CPT2 antibodies bridge basic research and clinical applications, enhancing understanding of mitochondrial metabolism and related pathologies.
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