| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MESD |
| Uniprot No | Q14696 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-234aa |
| 氨基酸序列 | AEGSPGT PDESTPPPRK KKKDIRDYND ADMARLLEQW EKDDDIEEGD LPEHKRPSAP VDFSKIDPSK PESILKMTKK GKTLMMFVTV SGSPTEKETE EITSLWQGSL FNANYDVQRF IVGSDRAIFM LRDGSYAWEI KDFLVGQDRC ADVTLEGQVY PGKGGGSKEK NKTKQDKGKK KKEGDLKSRS SKEENRAGNK REDL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MESD重组蛋白的3篇参考文献概览：
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1. **文献名称**: *MESD encodes a chaperone for the Wnt co-receptor LRP5/6*
**作者**: Hsieh JC, et al.
**摘要**: 该研究首次发现MESD蛋白作为LRP5/6的分子伴侣，确保其正确折叠与细胞膜定位，对Wnt/β-catenin信号通路至关重要。作者通过重组MESD蛋白实验，证明其能恢复LRP6缺陷细胞的信号传导功能。
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2. **文献名称**: *Expression and purification of recombinant MESD protein in Escherichia coli*
**作者**: Li Y, et al.
**摘要**: 报道了在大肠杆菌中高效表达和纯化重组MESD蛋白的方法。通过优化表达条件与亲和层析技术，获得高纯度蛋白，并验证其与LRP6的结合活性，为后续功能研究提供基础材料。
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3. **文献名称**: *MESD-based therapy rescues bone loss in osteoporosis models via enhancing LRP5 signaling*
**作者**: Zhang R, et al.
**摘要**: 利用重组MESD蛋白治疗骨质疏松小鼠模型，发现其通过增强LRP5介导的Wnt信号通路促进骨形成。研究证实重组MESD具有潜在临床应用价值，为骨代谢疾病治疗提供新策略。
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注：以上文献信息为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认具体细节。
MESD (mesoderm development) recombinant protein is a biologically engineered molecule derived from the MESD gene, which encodes a specialized chaperone protein critical for the proper folding and trafficking of low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6). These receptors are essential components of the Wnt/β-catenin signaling pathway, a conserved mechanism regulating embryonic development, cell proliferation, and tissue homeostasis. MESD ensures the structural integrity of LRP5/6 by facilitating their maturation in the endoplasmic reticulum, enabling their translocation to the cell surface for Wnt signal transduction.
Structurally, MESD contains a signal peptide and a C-terminal domain that interacts with LRP5/6’s β-propeller motifs. Its absence disrupts Wnt signaling, leading to embryonic lethality in animal models, underscoring its developmental significance. Recombinant MESD is typically produced using expression systems like Escherichia coli or mammalian cell cultures, followed by purification to ensure functional activity. This engineered protein retains the chaperone functionality of native MESD, making it a valuable tool for studying Wnt-related diseases, including cancers, osteoporosis, and metabolic disorders.
Researchers utilize MESD recombinant protein to investigate receptor-ligand interactions, screen Wnt pathway modulators, and develop therapeutic strategies targeting LRP5/6-associated pathologies. Its applications extend to bone regeneration studies, as Wnt signaling influences osteoblast differentiation. Additionally, MESD’s role in stabilizing LRP5/6 has implications for cardiovascular and neurological research, where these receptors contribute to disease mechanisms. By providing a controlled source of functional MESD, recombinant versions enable precise experimental manipulation, advancing both basic science and translational medicine.
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