| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | FKHL9; FOXD4A; FREAC5; FREAC-5 |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FOXD4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FOXD4抗体的3篇示例参考文献(内容为模拟，供参考)：
1. **文献名称**：*FOXD4 as a Novel Biomarker in Glioblastoma: Expression Analysis and Clinical Implications*
**作者**：Smith J, et al.
**摘要**：研究利用FOXD4抗体通过免疫组化分析胶质母细胞瘤组织，发现FOXD4高表达与患者预后不良相关，提示其可能作为潜在治疗靶点。
2. **文献名称**：*FOXD4 Antibody Validation for Neural Stem Cell Lineage Tracing*
**作者**：Lee H, et al.
**摘要**：本文验证了FOXD4抗体在小鼠模型中的特异性，证明其可用于标记神经干细胞分化过程，为研究神经发育机制提供工具。
3. **文献名称**：*Mechanistic Role of FOXD4 in Epithelial-Mesenchymal Transition Revealed by Antibody-Based Inhibition*
**作者**：Garcia R, et al.
**摘要**：通过FOXD4抗体阻断实验，发现FOXD4通过调控Snail信号通路促进乳腺癌细胞上皮间质转化(EMT)，影响转移进程。
4. **文献名称**：*Development of a High-Specificity FOXD4 Monoclonal Antibody for Western Blot Applications*
**作者**：Wang T, et al.
**摘要**：研究报道了一种新型FOXD4单克隆抗体的开发，验证其在Western Blot中的高特异性和低交叉反应性，适用于基础研究。
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**注意**：以上为示例文献，实际文献需通过PubMed、Google Scholar等平台检索关键词“FOXD4 antibody”或“FOXD4 function”获取。
The FOXD4 antibody is a research tool targeting the FOXD4 protein, a member of the Forkhead box (Fox) family of transcription factors. FOXD4 plays a critical role in embryonic development, particularly in regulating neural progenitor cell proliferation, differentiation, and neurogenesis. It is expressed during early neurodevelopment, where it helps maintain the balance between self-renewal and differentiation of neural stem cells. Dysregulation of FOXD4 has been implicated in neurodevelopmental disorders and certain cancers, making it a subject of interest in both developmental biology and oncology research.
The FOXD4 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect FOXD4 expression patterns in tissues or cultured cells. Researchers utilize it to study FOXD4's involvement in neural tube formation, brain development, and its potential role in tumorigenesis, such as in glioblastoma or neuroblastoma. Some studies also explore FOXD4's interaction with signaling pathways like Wnt or Notch.
Commercially available FOXD4 antibodies are typically validated for specificity and sensitivity, though results may vary depending on isoforms or post-translational modifications. Challenges include cross-reactivity with other Fox family members, necessitating careful experimental controls. Ongoing research continues to clarify FOXD4's functional mechanisms, aided by advanced tools like CRISPR or ChIP-seq, with the antibody remaining pivotal in visualizing FOXD4 dynamics across biological contexts.
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