Rabbit Polyclonal TBX2 Antibody

The TBX2 antibody is a crucial tool in studying the T-box transcription factor 2 (TBX2), a member of the T-box gene family involved in embryonic development and tissue homeostasis. TBX2 plays a key role in regulating organogenesis, particularly in heart, limb, and brain development, by modulating cell proliferation, differentiation, and patterning. It acts as a transcriptional repressor, often interacting with chromatin modifiers to suppress target genes like CDKN2A (p14ARF/p16INK4a), thereby influencing cell cycle progression and senescence. Dysregulation of TBX2 is linked to developmental disorders and cancers, including melanoma, breast, and pancreatic cancers, where its overexpression promotes tumorigenesis by inhibiting apoptosis and senescence. Researchers use TBX2 antibodies in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect TBX2 expression, localization, and interactions in cells or tissues. These antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown. Commercial TBX2 antibodies are available as monoclonal or polyclonal variants, with reactivity often spanning human, mouse, and rat samples. Their applications extend to developmental biology, cancer research, and regenerative medicine, aiding in understanding TBX2's dual roles in normal physiology and disease. Proper validation and optimization remain critical due to potential cross-reactivity with other T-box proteins like TBX3.