| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TDT |
| Entrez GeneID | 1791 |
| clone | 4B10C3 |
| WB Predicted band size | 58.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DNTT (AA: 52-192) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与IRS4抗体相关的示例文献(注：以下为假设性示例，实际文献需通过数据库查询验证)：
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1. **文献名称**: "IRS4 Expression in Prostate Cancer: Role in Androgen Receptor Signaling"
**作者**: Cheng, L. et al.
**摘要**: 研究利用特异性IRS4抗体检测前列腺癌组织中IRS4蛋白的表达，发现其高表达与雄激素受体信号通路激活相关，可能促进肿瘤细胞增殖。
2. **文献名称**: "Development and Validation of a Monoclonal Antibody Targeting Human IRS4"
**作者**: Smith, J.R. & Patel, R.
**摘要**: 描述了新型IRS4单克隆抗体的开发过程，通过Western blot和免疫荧光验证其特异性，并成功应用于多种癌细胞系的IRS4蛋白定位研究。
3. **文献名称**: "IRS4 Deficiency Alters Metabolic Homeostasis in Murine Models"
**作者**: Jones, M.K. et al.
**摘要**: 使用IRS4抗体分析IRS4敲除小鼠组织样本，发现IRS4缺失导致胰岛素信号通路异常，提示其在糖代谢调节中的潜在作用。
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建议通过PubMed或Google Scholar检索关键词“IRS4 antibody”、“IRS4 signaling”等获取真实文献。
The insulin receptor substrate 4 (IRS4) antibody is a research tool designed to detect and study the IRS4 protein, a member of the insulin receptor substrate family. IRS proteins are critical intracellular adaptors in insulin and insulin-like growth factor (IGF) signaling pathways. IRS4. encoded by the *IRS4* gene, shares structural features with other IRS proteins, including pleckstrin homology and phosphotyrosine-binding domains, which mediate interactions with activated receptors and downstream signaling molecules. However, IRS4 exhibits distinct tissue expression patterns, primarily in the brain, pituitary, and thyroid glands, suggesting specialized roles in neuroendocrine regulation or metabolism.
IRS4 antibodies are widely used in molecular biology to investigate IRS4’s function in cellular processes such as glucose homeostasis, cell growth, and differentiation. These antibodies enable detection of IRS4 expression levels, post-translational modifications (e.g., phosphorylation), and localization via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Researchers also employ IRS4 antibodies to explore its involvement in diseases, including diabetes, obesity, and cancers, where IRS4 dysregulation may contribute to insulin resistance or tumor progression.
Commercially available IRS4 antibodies are typically monoclonal or polyclonal, raised in hosts like rabbits or mice. Validation includes testing for specificity using knockout cell lines or tissues. Challenges remain in ensuring antibody selectivity due to structural similarities among IRS family members. Nonetheless, IRS4 antibodies remain vital for unraveling the protein’s unique signaling roles and therapeutic potential.
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