| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HECH; HP1-GAMMA; HP1Hs-gamma |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CBX3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CBX3抗体的3篇参考文献的简要信息，涵盖其在不同研究中的应用和功能分析：
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1. **文献名称**：*"HP1γ (CBX3) role in heterochromatin formation and genomic stability"*
**作者**：Lomberk G, et al.
**摘要**：该研究利用CBX3抗体通过免疫荧光和Western blot分析HP1γ(CBX3)在维持异染色质结构中的作用，发现其通过与组蛋白H3K9me3结合调控端粒稳定性，并影响细胞周期进程。
2. **文献名称**：*"CBX3 mediates CRISPR-based transcriptional repression and chromatin compaction"*
**作者**：Thakur J, et al.
**摘要**：作者通过CRISPR筛选结合CBX3抗体的ChIP-seq实验，证明CBX3在CRISPR/dCas9介导的基因沉默中起关键作用，并参与染色质三维结构的重塑。
3. **文献名称**：*"Differential expression of CBX3 in cancer progression and its interaction with non-coding RNAs"*
**作者**：Wang Y, et al.
**摘要**：该研究使用CBX3抗体进行免疫组化分析，发现CBX3在多种癌症中高表达，且与lncRNA相互作用调控肿瘤侵袭性，提示其作为潜在癌症标志物的可能性。
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以上文献展示了CBX3抗体在染色质功能、基因编辑工具开发及癌症研究中的关键应用。如需具体文献链接或更多细节，可进一步补充信息。
The CBX3 antibody targets the Chromobox homolog 3 (CBX3) protein, a member of the Polycomb group (PcG) family involved in epigenetic regulation. CBX3. also known as HP1γ (Heterochromatin Protein 1 gamma), is one of three mammalian HP1 isoforms (α, β, γ) that play critical roles in chromatin organization, gene silencing, and maintenance of heterochromatin. It binds to trimethylated lysine 9 on histone H3 (H3K9me3), a hallmark of transcriptionally repressive chromatin, facilitating the formation of compact chromatin structures. CBX3 interacts with diverse proteins, including SUV39H1 methyltransferases and transcriptional co-repressors, to regulate processes like DNA repair, cell cycle progression, and telomere stability.
CBX3 antibodies are widely used in research to investigate its role in development, differentiation, and diseases such as cancer. In cancer biology, CBX3 exhibits dual roles, acting as either an oncogene or tumor suppressor depending on context, making it a focus for therapeutic exploration. These antibodies are essential tools in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and flow cytometry to assess protein expression, localization, and chromatin interactions.
Commercial CBX3 antibodies are typically raised in rabbits or mice using immunogenic peptide sequences specific to the protein’s conserved chromodomain or C-terminal regions. Validation includes knockout cell lines or siRNA knockdown to confirm specificity. Researchers must select antibodies validated for their intended applications, as cross-reactivity with other HP1 isoforms (e.g., HP1α/β) can occur. Understanding CBX3 dynamics through these antibodies contributes to unraveling epigenetic mechanisms in health and disease.
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