| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | AFBP, IBP1, PP12, IGF-BP25, hIGFBP-1 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IGFBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IGFBP1抗体的示例参考文献(内容为示例，非真实文献)：
1. **《Development of a monoclonal antibody for human IGFBP1 detection in gestational diabetes》**
- 作者：Smith A, et al.
- 摘要：研究开发了一种高特异性小鼠单克隆抗体，用于ELISA检测孕妇血清中的IGFBP1水平，发现其与妊娠糖尿病风险显著相关。
2. **《IGFBP1 expression in hepatocellular carcinoma: Immunohistochemical analysis using a novel antibody》**
- 作者：Lee JH, et al.
- 摘要：通过新型兔多克隆抗体进行免疫组化实验，揭示肝癌组织中IGFBP1表达下调，提示其可能作为肿瘤抑制因子。
3. **《Role of IGFBP1 in insulin resistance: Neutralizing antibody studies in vitro》**
- 作者：Brown K, et al.
- 摘要：利用中和抗体阻断IGFBP1功能，证明其在脂肪细胞胰岛素信号通路中的调控作用，为代谢疾病机制提供依据。
4. **《A polyclonal anti-IGFBP1 antibody for cross-species reactivity validation》**
- 作者：Zhang Y, et al.
- 摘要：报道了一种兔源多克隆抗体，验证其在小鼠、大鼠及人类样本中的交叉反应性，适用于Western blot和免疫荧光检测。
建议通过PubMed或Google Scholar搜索真实文献，关键词如“IGFBP1 antibody”、“IGFBP1 detection”或结合具体研究领域(如妊娠、癌症)。
Insulin-like growth factor-binding protein 1 (IGFBP1) is a member of the IGFBP family that modulates the bioavailability and activity of insulin-like growth factors (IGFs), which are critical regulators of cell growth, metabolism, and differentiation. IGFBP1 primarily binds IGF-1 and IGF-2. sequestering them in circulation and limiting their interaction with cell surface receptors. This protein is predominantly expressed in the liver and decidualized endometrium, with its expression influenced by metabolic and hormonal signals, including insulin, glucagon, and hypoxia.
Antibodies targeting IGFBP1 are essential tools for studying its role in physiological and pathological processes. They are widely used in immunoassays (e.g., ELISA, Western blotting) and immunohistochemistry to quantify protein levels, assess tissue localization, or investigate post-translational modifications like phosphorylation, which alters IGF-binding affinity. Research applications include exploring IGFBP1's involvement in pregnancy (e.g., placental development, preeclampsia), metabolic disorders (e.g., insulin resistance, gestational diabetes), and cancer (e.g., tumor proliferation, metastasis).
Clinically, IGFBP1 antibodies have diagnostic potential, particularly in obstetrics, where low maternal serum IGFBP1 levels may indicate fetal growth restriction. Additionally, they aid in understanding IGFBP1's dual role as both an IGF antagonist and a cell-adhesion molecule through integrin interactions. Species cross-reactivity (e.g., human, mouse, rat) is often validated to support translational studies. Robust validation of these antibodies ensures specificity, enabling accurate mechanistic insights into IGF axis regulation and therapeutic targeting.
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