| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | A1B; ABG; GAB; HYST2477; DKFZp686F0970; A1BG |
| Entrez GeneID | 1 |
| clone | 4B5 |
| WB Predicted band size | 54kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human A1BG expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAP4K1抗体的3篇参考文献概览(均为虚构示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**: "MAP4K1 modulates T cell receptor signaling through phosphorylation of SLP-76"
**作者**: Smith, J. et al.
**摘要**: 本研究利用特异性MAP4K1抗体，揭示了MAP4K1在T细胞活化中通过磷酸化SLP-76调控TCR信号通路的机制，为免疫调节提供新靶点。
2. **文献名称**: "Targeting MAP4K1 with a monoclonal antibody inhibits tumor growth in melanoma models"
**作者**: Chen, L. et al.
**摘要**: 开发了一种高亲和力抗MAP4K1单克隆抗体，证实其通过阻断MAP4K1与JNK通路的相互作用，显著抑制黑色素瘤小鼠模型的肿瘤进展。
3. **文献名称**: "Development and validation of a rabbit polyclonal antibody against human MAP4K1 for functional studies"
**作者**: Wang, Y. et al.
**摘要**: 报道了一种兔源多克隆抗体的制备与验证，该抗体能特异性识别MAP4K1蛋白，适用于免疫印迹和免疫组化，助力MAP4K1在多种疾病中的功能研究。
建议通过 **PubMed/Google Scholar** 搜索实际文献，结合关键词“MAP4K1 antibody”、“MAP4K1 function”或“HPK1 (MAP4K1别名)”获取准确信息。
MAP4K1 (mitogen-activated protein kinase kinase kinase kinase 1), also known as hematopoietic progenitor kinase 1 (HPK1), is a serine/threonine kinase belonging to the STE20 family. It plays a critical role in immune regulation and cellular signaling, particularly in T-cell receptor (TCR) and B-cell receptor (BCR) pathways. Structurally, MAP4K1 contains an N-terminal kinase domain, a central coiled-coil domain for protein interactions, and a C-terminal citron homology (CNH) domain. It functions as a negative regulator of T-cell activation by promoting the degradation of the adaptor protein SLP-76. thereby modulating immune responses. Dysregulation of MAP4K1 has been implicated in autoimmune diseases, chronic inflammation, and cancer progression.
Antibodies targeting MAP4K1 are essential tools for studying its expression, localization, and function in various biological contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to investigate MAP4K1's role in immune cell signaling, apoptosis, and stress responses. Researchers also utilize these antibodies to explore MAP4K1's therapeutic potential, as its inhibition has shown promise in enhancing anti-tumor immunity and alleviating autoimmune conditions. Both monoclonal and polyclonal MAP4K1 antibodies are available, often developed in hosts like rabbits or mice, with validation in specific applications. Understanding MAP4K1's molecular mechanisms through antibody-based studies continues to advance drug discovery efforts targeting immune-related diseases.
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