| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | APC4 |
| WB Predicted band size | 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ANAPC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ANAPC4抗体的3-4篇参考文献的示例(注：文献信息为示例性质，可能需根据实际研究调整)：
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1. **标题**: *APC/C Subunit ANAPC4 is Essential for Cell Cycle Progression*
**作者**: Yamamoto S, et al.
**摘要**: 研究利用ANAPC4抗体进行免疫印迹和免疫荧光实验，发现敲除ANAPC4会导致细胞周期停滞在G1期，证实其在APC/C复合体介导的泛素化降解中的核心作用。
2. **标题**: *Structural and Functional Analysis of ANAPC4 in the APC/C Complex*
**作者**: Zhang M, et al.
**摘要**: 通过ANAPC4抗体进行免疫共沉淀和质谱分析，揭示了ANAPC4与其他APC/C亚基的相互作用，阐明其维持复合体结构完整性的关键功能。
3. **标题**: *ANAPC4 Dysregulation in Cancer: A Role in Genomic Instability*
**作者**: Chen L, et al.
**摘要**: 使用ANAPC4抗体检测肿瘤组织样本，发现其表达水平与染色体不稳定性相关，提示ANAPC4缺失可能通过破坏APC/C功能促进癌症进展。
4. **标题**: *ANAPC4 Antibody-based Screening for Drug Resistance in Yeast*
**作者**: Gupta P, et al.
**摘要**: 开发了一种基于ANAPC4抗体的高通量筛选方法，发现靶向APC/C活性的化合物可逆转酵母模型中的化疗耐药表型。
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**注意**：以上文献为示例，实际引用需根据具体研究检索PubMed、Google Scholar等数据库获取。推荐使用关键词“ANAPC4 antibody”、“APC/C complex”或结合具体研究领域(如癌症、细胞周期)进行精准搜索。
The ANAPC4 antibody targets the Anaphase-Promoting Complex subunit 4 (ANAPC4), a critical component of the Anaphase-Promoting Complex/Cyclosome (APC/C). APC/C is a multi-subunit E3 ubiquitin ligase that regulates cell cycle progression by targeting specific proteins for proteasomal degradation, ensuring timely transitions through key phases like metaphase-to-anaphase and exit from mitosis. ANAPC4. also known as APC4. plays a structural and regulatory role in maintaining APC/C stability and substrate recognition. It facilitates interactions between the core complex and co-activators (CDC20 or CDH1), which are essential for activating APC/C's ubiquitination activity toward substrates such as cyclin B1 and securin.
ANAPC4 antibodies are widely used in research to study cell cycle dynamics, protein degradation mechanisms, and mitotic regulation. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ANAPC4 expression, localization, and interactions in various biological contexts. Dysregulation of APC/C function, including ANAPC4 abnormalities, has been linked to cancers, genomic instability, and developmental disorders. Researchers also use these antibodies to explore therapeutic targets, as modulating APC/C activity could influence cell proliferation in diseases like cancer. Validation of ANAPC4 antibodies often involves knockout cell lines or tissue models to confirm specificity, ensuring reliable detection in both physiological and pathological studies.
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