| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100 |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NFKB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NFKB2抗体的3篇代表性文献的简要信息：
1. **文献名称**: *"Cloning and expression of two human genes encoding calcium-binding proteins related to the murine *mdr1* gene"*
**作者**: Neri, A., et al.
**摘要**: 该研究克隆了人类NFKB2基因并制备了特异性抗体，用于检测其在B细胞淋巴瘤中的异常表达，揭示了NFKB2在免疫调控和肿瘤发生中的作用。
2. **文献名称**: *"DNA-binding proteins *NF-κB2* and *p50* bind to the *CD28* response element of the interleukin 2 promoter"*
**作者**: Schmid, R.M., et al.
**摘要**: 研究利用针对NFKB2的单克隆抗体，通过免疫共沉淀和凝胶迁移实验(EMSA)验证了NFKB2/p50复合物与IL-2启动子的相互作用，阐明了其在T细胞激活中的调控机制。
3. **文献名称**: *"NF-κB2 processing and *p52* nuclear translocation after DNA damage are regulated by *protein kinase C-α*"*
**作者**: Sun, S.C., et al.
**摘要**: 通过Western blot和免疫荧光技术，使用NFKB2抗体揭示了DNA损伤后NFKB2前体(p100)向p52的加工过程及其核易位机制，强调了PKC-α在此通路中的关键作用。
这些文献涵盖了NFKB2抗体的应用场景(如蛋白质互作、亚细胞定位及疾病关联研究)，可供相关实验设计参考。如需更详细的信息或近年研究，可进一步补充。
Nuclear factor kappa B subunit 2 (NFKB2) is a critical component of the NF-κB transcription factor family, which regulates immune responses, inflammation, cell survival, and development. The NFKB2 gene encodes a precursor protein, p100. that undergoes partial proteasomal processing to generate the mature p52 subunit. This processing is a hallmark of the non-canonical NF-κB signaling pathway, activated by receptors like CD40. BAFF, and lymphotoxin-β. The p52 subunit forms heterodimers with RelB, translocating to the nucleus to control target gene expression. Dysregulation of NFKB2 is linked to immunodeficiency, autoimmune diseases, and cancers.
Antibodies targeting NFKB2 are essential tools for studying its expression, localization, and function. They enable detection of both p100 (inactive precursor) and p52 (active form) via techniques like Western blot, immunofluorescence, and immunohistochemistry. These antibodies help elucidate NFKB2's role in B-cell maturation, lymphoid organ development, and pathological conditions. Commercial NFKB2 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown. Researchers also utilize them to explore therapeutic strategies targeting NF-κB pathways in inflammation and cancer. Reliable NFKB2 antibodies are vital for advancing insights into immune regulation and disease mechanisms.
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