| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PP2CB, PP2Abeta |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PPP2CB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP2CB抗体的示例参考文献(内容为模拟，供参考)：
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1. **文献名称**: *"PP2A Catalytic Subunit PPP2CB as a Novel Biomarker in Colorectal Cancer"*
**作者**: Jespersen, T. et al. (2021)
**摘要**: 研究通过免疫组化和Western blot分析，发现PPP2CB蛋白在结直肠癌组织中表达显著下调，并验证了其抗体的特异性。结果表明PPP2CB可能作为肿瘤抑制因子参与癌症进展。
2. **文献名称**: *"Subcellular Localization of PPP2CB in Neuronal Cells Using a Novel Monoclonal Antibody"*
**作者**: Smith, R. et al. (2018)
**摘要**: 开发了一种高特异性抗PPP2CB的单克隆抗体，用于免疫荧光和共聚焦显微镜分析，揭示了PPP2CB在神经元突触中的定位，提示其在神经信号传导中的作用。
3. **文献名称**: *"Regulation of PP2A Activity by Phosphorylation: Insights from PPP2CB-Specific Antibody Studies"*
**作者**: Brautigan, D.L. & Virshup, D.M. (2017)
**摘要**: 通过磷酸化特异性PPP2CB抗体，研究发现酪氨酸307位点的磷酸化可抑制PP2A酶活性，为癌症和代谢疾病中PP2A功能失调提供了机制解释。
4. **文献名称**: *"PPP2CB Antibody Validation for Viral Infection Research"*
**作者**: Nguyen, H. et al. (2020)
**摘要**: 验证了多种PPP2CB抗体在病毒感染模型中的适用性，发现特定抗体能有效检测病毒蛋白与PP2A复合物的相互作用，提示PPP2CB在宿主-病原体互作中的潜在作用。
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**备注**：以上文献为示例性内容，实际引用请通过PubMed、Google Scholar等平台检索真实文献(关键词：PPP2CB antibody, PP2A catalytic subunit, protein phosphatase 2A)。
The PPP2CB antibody targets the catalytic subunit (Cα) of Protein Phosphatase 2A (PP2A), a ubiquitously expressed serine/threonine phosphatase critical for cellular processes such as signal transduction, cell cycle regulation, and apoptosis. PP2A exists as a heterotrimeric enzyme composed of a structural (A), regulatory (B), and catalytic (C) subunit. PPP2CB encodes the Cα isoform, which binds the A subunit to form the core enzyme, while B subunits determine substrate specificity and subcellular localization.
PPP2CB-mediated dephosphorylation regulates key signaling pathways, including Wnt, MAPK, and PI3K/AKT, maintaining cellular homeostasis. Dysregulation of PP2A activity is linked to cancers, neurodegenerative disorders, and cardiovascular diseases. The PPP2CB antibody is widely used in research to study PP2A expression, activity, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. It helps elucidate PP2A's tumor-suppressive roles, such as inactivating oncogenic kinases or stabilizing tumor suppressors like p53.
Commercial PPP2CB antibodies are typically raised against specific epitopes (e.g., C-terminal regions) and validated for cross-reactivity across species (human, mouse, rat). Researchers rely on these antibodies to explore PP2A dysfunction in disease models or screen therapeutic agents targeting phosphatase activity. Careful validation is essential, as PP2A subunit composition and post-translational modifications (e.g., methylation, phosphorylation) can affect antibody specificity.
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