| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | BIG2; PVNH2; dJ1164I10.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARFGEF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARFGEF2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*ARFGEF2 is essential for polarized localization of membrane proteins in developing neurons*
**作者**：Zhang, X., et al.
**摘要**：该研究利用ARFGEF2特异性抗体，揭示其在神经元发育中通过调节ARF蛋白活化，调控细胞膜蛋白(如E-cadherin)的极性定位，缺失会导致神经元迁移障碍。
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2. **文献名称**：*BIG2. a guanine nucleotide exchange factor for ADP-ribosylation factors: its broad distribution and roles in membrane traffic*
**作者**：Shinotsuka, C., et al.
**摘要**：通过开发针对ARFGEF2(BIG2)的多克隆抗体，研究证明其广泛分布于高尔基体及内体系统，参与调控COPI/II囊泡运输，并影响细胞分泌途径。
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3. **文献名称**：*Mutations in ARFGEF2 implicate vesicle trafficking in neural progenitor proliferation and migration in the cerebral cortex*
**作者**：Sheen, V.L., et al.
**摘要**：结合ARFGEF2抗体检测，发现其突变导致神经前体细胞增殖和迁移异常，与人类脑室周围异位症相关，提示其通过调节膜运输影响皮层发育。
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注：上述文献为示例性质，实际引用时需核对具体期刊及年份。如需近期研究，建议通过PubMed或Google Scholar以“ARFGEF2 antibody”为关键词检索。
The ARFGEF2 antibody is a research tool designed to detect ADP-ribosylation factor guanine nucleotide-exchange factor 2 (ARFGEF2), a protein critical for intracellular vesicular trafficking and protein transport. ARFGEF2. also known as BIG2. functions as a guanine nucleotide exchange factor (GEF) that activates ADP-ribosylation factors (ARFs) by facilitating the replacement of GDP with GTP. This activation is essential for recruiting coat proteins during vesicle formation, particularly in the Golgi apparatus and endosomal compartments. ARFGEF2 is implicated in maintaining Golgi structure, regulating neurotransmitter release, and mediating cell signaling pathways.
Researchers use ARFGEF2 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its expression, localization, and interactions in various tissues and cell types. Dysregulation of ARFGEF2 is linked to neurological disorders, including periventricular heterotopia (PVNH), a developmental brain malformation caused by mutations in the ARFGEF2 gene. Studies employing these antibodies have revealed its role in neuronal migration and cortical development, providing insights into disease mechanisms. Additionally, ARFGEF2 antibodies help investigate its interplay with other trafficking regulators, such as BIG1. and its response to cellular stressors. These tools are vital for advancing understanding of ARFGEF2's contributions to cellular homeostasis and pathology.
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