| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | APG4B; AUTL1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ATG4B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ATG4B抗体的3篇代表性文献的简要总结：
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1. **"A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors"**
*作者：Li et al. (2019)*
**摘要**：研究开发了一种特异性靶向ATG4B的小分子抑制剂，并利用ATG4B抗体验证其对自噬通路的抑制作用。实验表明，抑制ATG4B可阻断LC3蛋白的加工，抑制肿瘤细胞生长，为骨肉瘤治疗提供新策略。
2. **"Characterization of monoclonal antibodies against ATG4B and their application in autophagy detection"**
*作者：Tanida et al. (2008)*
**摘要**：该文献报道了针对人源ATG4B蛋白的单克隆抗体制备，验证了抗体在Western blot和免疫荧光中的特异性。研究证实ATG4B在自噬过程中对LC3前体的切割起关键作用，抗体可用于动态监测自噬活性。
3. **"ATG4B regulates autophagic flux and mitochondrial homeostasis in colorectal cancer cells"**
*作者：Marino et al. (2020)*
**摘要**：通过ATG4B抗体及基因敲除技术，研究发现ATG4B缺失导致自噬流中断和线粒体功能障碍，强调了其在结直肠癌细胞存活中的必要性，为靶向自噬的癌症治疗提供了依据。
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以上文献涵盖了ATG4B抗体的开发、功能验证及在疾病机制研究中的应用。如需具体文献来源，建议通过PubMed或Web of Science检索标题或作者进一步获取全文。
ATG4B (Autophagy-related protein 4 homolog B) is a cysteine protease essential for autophagy, a cellular degradation process critical for maintaining homeostasis. It specifically cleaves the C-terminal region of microtubule-associated protein 1 light chain 3 (LC3), enabling its conjugation to phosphatidylethanolamine (PE) during autophagosome formation. This post-translational modification, termed LC3 lipidation, is pivotal for autophagosome membrane expansion and cargo recruitment. ATG4B also delipidates LC3-PE to recycle LC3. ensuring dynamic membrane remodeling. Dysregulation of ATG4B is linked to diseases such as cancer, neurodegenerative disorders, and infections, making it a therapeutic target.
ATG4B antibodies are vital tools for studying autophagy mechanisms. They detect endogenous ATG4B protein levels via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Researchers use these antibodies to investigate ATG4B expression patterns, subcellular localization, and interactions with autophagy regulators (e.g., ATG7. LC3). Commercial ATG4B antibodies are typically raised against specific epitopes, often validated using knockout cell lines or siRNA-mediated silencing to confirm specificity. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies ensure consistency. Recent studies also employ ATG4B-targeting antibodies to explore its role in modulating chemotherapeutic responses or neurodegenerative protein aggregation. Validation parameters include species reactivity, application compatibility, and cross-reactivity checks to avoid off-target signals.
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