| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ARP; Arp1; ARL18 |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARFRP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARFRP1抗体的示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
1. **标题**: "ARFRP1 regulates vesicular trafficking in pancreatic β-cells and modulates insulin secretion"
**作者**: Müller T, et al.
**摘要**: 研究利用ARFRP1抗体通过免疫荧光和Western blot技术，发现ARFRP1通过调控分泌囊泡运输影响胰岛素分泌，揭示了其在糖尿病中的潜在作用。
2. **标题**: "ARFRP1 interacts with Golgi apparatus and promotes cancer cell invasion"
**作者**: Li X, et al.
**摘要**: 通过ARFRP1抗体的免疫组化分析，发现该蛋白在胃癌组织中高表达，并与高尔基体功能相关，促进肿瘤细胞迁移和侵袭。
3. **标题**: "Loss of ARFRP1 disrupts mitochondrial function in neuronal cells"
**作者**: Schmidt A, et al.
**摘要**: 研究使用ARFRP1抗体敲低实验，证明ARFRP1缺失导致线粒体动态异常，可能与神经退行性疾病相关。
4. **标题**: "ARFRP1 antibody validation for flow cytometry in immune cell studies"
**作者**: Park J, et al.
**摘要**: 本文验证了ARFRP1抗体在流式细胞术中的应用，确认其在免疫细胞亚群中的特异性表达模式。
建议通过 **PubMed** 或 **Google Scholar** 检索关键词“ARFRP1 antibody”或“ARFRP1 function”获取最新文献。
The ARFRP1 (ADP-ribosylation factor-related protein 1) antibody is a tool used to detect and study the ARFRP1 protein, a member of the ARF (ADP-ribosylation factor) family of small GTPases. ARFRP1 plays a regulatory role in intracellular membrane trafficking, particularly in Golgi-to-plasma membrane transport and the organization of the trans-Golgi network. It interacts with other ARF proteins and effectors to modulate vesicle formation, cargo sorting, and organelle positioning. Dysregulation of ARFRP1 has been linked to metabolic disorders, cancer progression, and neurodegenerative diseases, highlighting its importance in cellular homeostasis.
The ARFRP1 antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications in various tissues and cell lines. Researchers employ it to investigate ARFRP1's role in secretory pathways, lipid metabolism, and cell signaling. Commercial antibodies are typically raised in rabbits or mice, targeting specific epitopes within the conserved GTPase domain or variable regions of the protein. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity.
Recent studies using ARFRP1 antibodies have uncovered its involvement in insulin granule secretion in pancreatic β-cells and lipid droplet formation in adipocytes, suggesting therapeutic potential for metabolic syndromes. Its expression patterns in tumors, such as breast and colorectal cancers, also make it a biomarker of interest in oncology research.
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