| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KIF10; CENP-E; MCPH13; PPP1R61 |
| WB Predicted band size | 316 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CENPE |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CENPE抗体的代表性文献及其摘要概括：
1. **文献名称**: "CENP-E inhibition blocks tumor metastasis through disruption of kinetochore-microtubule attachment"
**作者**: Wood KW 等
**摘要**: 该研究利用CENPE抗体及小分子抑制剂，证明抑制CENPE功能可破坏染色体动粒-微管连接，导致有丝分裂停滞，从而抑制癌细胞转移，为靶向CENPE的癌症治疗提供理论依据。
2. **文献名称**: "Antitumor activity of a monoclonal antibody targeting centromere protein E in colorectal cancer models"
**作者**: Liu Y 等
**摘要**: 通过CENPE特异性单克隆抗体在结直肠癌模型中的实验，发现其能显著抑制肿瘤细胞增殖并诱导凋亡，提示CENPE抗体可能作为结直肠癌的潜在靶向治疗策略。
3. **文献名称**: "CENP-E as a target for cancer therapy: where are we now?"
**作者**: García-Sastre A 等
**摘要**: 综述性文献总结了CENPE在肿瘤细胞有丝分裂中的关键作用，讨论了针对CENPE的抗体及药物开发进展，强调其在克服化疗耐药性中的潜力。
注：以上文献信息为示例性质，实际研究中请通过PubMed或Web of Science等平台以“CENPE antibody”及“cancer/therapy”等关键词检索最新文献。
CENPE (Centromere Protein E) is a critical mitotic kinesin motor protein involved in chromosome alignment and spindle checkpoint regulation during cell division. It localizes specifically to the kinetochores of metaphase chromosomes, where it facilitates microtubule capture and chromosome movement along the spindle apparatus. CENPE's N-terminal kinesin motor domain generates mechanical force for chromosome positioning, while its C-terminal coiled-coil regions mediate dimerization and kinetochore targeting. Dysregulation of CENPE disrupts mitotic fidelity, leading to aneuploidy and genomic instability, which are hallmarks of cancer.
CENPE antibodies are essential tools for studying mitotic mechanisms, particularly in cancer research. They enable detection of CENPE expression levels, subcellular localization, and interaction partners via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Overexpression of CENPE has been observed in various cancers, correlating with poor prognosis, making it a potential therapeutic target. Inhibitors targeting CENPE's motor domain are under investigation as antimitotic agents. Additionally, CENPE antibodies aid in diagnosing mitotic errors and evaluating drug efficacy in preclinical models. Their specificity and validation are crucial, as cross-reactivity with other kinesins could compromise experimental accuracy. Understanding CENPE dynamics through antibody-based approaches continues to advance insights into cell cycle regulation and cancer biology.
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