| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | GAT1; GABATR; GABATHG |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human SLC6A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SLC6A1抗体的3篇参考文献示例(注：文献为虚构示例，实际引用请查询真实数据库)：
1. **文献名称**: "Immunolocalization of SLC6A1 in the mammalian brain using a novel monoclonal antibody"
**作者**: Madsen et al.
**摘要**: 本研究开发了一种针对SLC6A1蛋白的高特异性单克隆抗体，通过免疫组化和Western blot验证其在啮齿类及人类脑组织中的表达，确认其在GABA能神经元突触前膜的高丰度分布。
2. **文献名称**: "Functional characterization of SLC6A1 mutations associated with epilepsy using antibody-based assays"
**作者**: Qian et al.
**摘要**: 通过抗体验证SLC6A1突变体的细胞膜定位缺陷，结合免疫荧光和流式细胞术，揭示突变导致GABA转运蛋白功能丧失的分子机制。
3. **文献名称**: "SLC6A1 antibody validation for comparative proteomic analysis in neurodevelopmental disorders"
**作者**: Rees et al.
**摘要**: 系统评估了多种商业SLC6A1抗体的特异性，优化了免疫沉淀和质谱联用方法，用于自闭症患者脑脊液中SLC6A1蛋白的定量分析。
如需真实文献，建议在PubMed或Google Scholar中检索关键词“SLC6A1 antibody validation”“SLC6A1 immunohistochemistry”等。
SLC6A1. also known as the sodium- and chloride-dependent GABA transporter 1 (GAT1), is a protein encoded by the SLC6A1 gene. It belongs to the solute carrier family 6 (SLC6) of neurotransmitter transporters and plays a critical role in regulating synaptic levels of γ-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. By mediating GABA reuptake from the synaptic cleft into presynaptic neurons and glial cells, SLC6A1 ensures precise control of GABAergic signaling, which is essential for maintaining neuronal excitability and preventing neurological hyperexcitability disorders.
Antibodies targeting SLC6A1 are widely used in neuroscience research to study the expression, localization, and function of this transporter. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize SLC6A1 distribution in brain tissues, cellular models, or experimental organisms. Specificity and validation of SLC6A1 antibodies are crucial, as cross-reactivity with related transporters (e.g., GAT2. GAT3) may occur.
Mutations in SLC6A1 are linked to neurodevelopmental disorders, including epilepsy, autism spectrum disorder, and intellectual disability. Research using SLC6A1 antibodies has advanced understanding of disease mechanisms, such as altered GABA homeostasis in SLC6A1-related encephalopathies. Additionally, these antibodies aid in evaluating therapeutic strategies targeting GABA transport, such as inhibitor drugs or gene therapies. Reliable SLC6A1 antibodies are thus vital tools for both basic research and translational studies in neuropharmacology and neuropathology.
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