| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | HHL; HRY; HES-1; bHLHb39 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HES1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HES1抗体的参考文献示例(注：以下文献信息为模拟示例，具体文献需通过学术数据库查询)：
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1. **"HES1 oscillations regulate neural stem cell self-renewal and differentiation"**
*作者：S. Hirano et al.*
摘要：研究通过免疫荧光和Western blot技术，利用HES1抗体检测神经干细胞中HES1蛋白的振荡表达，发现其动态变化与干细胞维持及分化密切相关，为Notch信号通路调控神经发生提供证据。
2. **"HES1 as a downstream target of Notch signaling in colorectal cancer"**
*作者：J. Li et al.*
摘要：通过免疫组化和Western blot分析，使用HES1抗体验证结直肠癌组织中HES1蛋白高表达，并证明其与Notch通路激活及肿瘤进展相关，提示HES1可能作为治疗靶点。
3. **"ChIP-seq analysis of HES1 binding sites in embryonic development"**
*作者：K. Nakamura & T. Honjo*
摘要：采用HES1抗体进行染色质免疫沉淀(ChIP-seq)，鉴定胚胎发育过程中HES1结合的DNA靶序列，揭示其在抑制神经分化基因中的直接调控作用。
4. **"Validation of a novel monoclonal HES1 antibody for flow cytometry applications"**
*作者：M. Tanaka et al.*
摘要：报道一种高特异性HES1单克隆抗体的开发与验证，应用于流式细胞术和免疫印迹，证明其在多种细胞系中检测HES1蛋白的可靠性和灵敏度。
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如需具体文献，建议通过PubMed、Google Scholar等平台以关键词“HES1 antibody”或“HES1 protein detection”检索，并筛选实验方法学或相关机制研究论文。
The HES1 antibody is a crucial tool for studying the HES1 protein, a basic helix-loop-helix (bHLH) transcription factor encoded by the *HES1* gene. HES1 acts as a downstream effector of the Notch signaling pathway, playing pivotal roles in developmental processes, including neurogenesis, somitogenesis, and tissue patterning. It functions as a transcriptional repressor by binding to N-box promoter regions of target genes, regulating cell differentiation, proliferation, and fate decisions. Dysregulation of HES1 is implicated in cancers, stem cell maintenance, and diseases like neurological disorders, making it a key focus in developmental biology and oncology research.
HES1 antibodies are widely used to detect and quantify HES1 expression in various applications, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies are typically raised in hosts like rabbits or mice, with monoclonal versions offering high specificity. Validated antibodies often demonstrate cross-reactivity across species such as human, mouse, and rat. Commercial suppliers (e.g., Cell Signaling Technology, Abcam) provide antibodies tested for sensitivity and specificity, often verified via knockout controls. Researchers rely on HES1 antibodies to explore its role in cellular dynamics, developmental pathways, and disease mechanisms, particularly in contexts where Notch signaling or stem cell pluripotency is disrupted. Proper validation ensures reliable results in both basic research and clinical studies.
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