| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | IDB4; bHLHb27 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ID4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ID4抗体的3篇模拟参考文献示例，涵盖不同研究方向和疾病模型：
1. **文献名称**：*"ID4 Expression in Triple-Negative Breast Cancer: Correlation with Clinical Outcomes Using a Novel Monoclonal Antibody"*
**作者**：Chen H, et al.
**摘要**：本研究开发了一种高特异性ID4单克隆抗体，用于检测三阴性乳腺癌组织中的ID4蛋白表达。结果显示ID4高表达与患者总生存期缩短显著相关，提示其可能作为预后不良的分子标志物。
2. **文献名称**：*"Epigenetic Silencing of ID4 in Colorectal Cancer via DNA Methylation: Validation by Antibody-Based Detection"*
**作者**：Martinez R, et al.
**摘要**：通过ID4抗体免疫组化分析结合甲基化测序，发现结直肠癌中ID4启动子区域高频甲基化导致蛋白表达缺失。功能实验表明ID4可通过抑制Wnt/β-catenin通路减缓肿瘤转移。
3. **文献名称**：*"Development of a Rabbit Polyclonal Antibody for ID4 and Its Application in Glioblastoma Stem Cell Research"*
**作者**：Kim S, et al.
**摘要**：报道一种新型兔源ID4多克隆抗体的制备及验证。该抗体成功用于胶质母细胞瘤干细胞模型，证实ID4通过维持干细胞自我更新能力促进肿瘤耐药性，靶向ID4可增强化疗敏感性。
---
注：上述文献为模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。若需具体文章，可提供疾病类型或研究场景进一步定向模拟。
ID4 (Inhibitor of DNA Binding 4) is a member of the ID protein family, which regulates cell differentiation and proliferation by modulating transcriptional activity. ID proteins lack a DNA-binding domain but interact with basic helix-loop-helix (bHLH) transcription factors, thereby inhibiting their ability to bind DNA and regulate target genes. ID4. specifically, is implicated in diverse biological processes, including cell cycle control, stem cell maintenance, and tissue development. Dysregulation of ID4 has been linked to cancer, where it often exhibits dual roles as either a tumor suppressor or oncogene, depending on context. For instance, ID4 is frequently downregulated in cancers like breast, ovarian, and prostate cancer due to promoter hypermethylation, correlating with poor prognosis. Conversely, in glioblastoma and certain hematologic malignancies, ID4 overexpression promotes tumor growth and therapy resistance.
Research on ID4 antibodies has focused on understanding its expression patterns, interactions, and functional roles. These antibodies are critical tools for detecting ID4 in tissues or cell lines via techniques like Western blotting, immunohistochemistry, or flow cytometry. Recent studies explore ID4's involvement in epigenetic regulation, such as modulating DNA methylation or histone modifications, and its crosstalk with signaling pathways like TGF-β and Wnt. Additionally, ID4 is studied in developmental contexts, particularly in neurogenesis and organogenesis.
Therapeutic targeting of ID4 remains exploratory, with potential strategies including epigenetic reactivation in ID4-silenced cancers or inhibition in ID4-driven malignancies. Its context-dependent roles underscore the need for precise biomarker-driven approaches in clinical applications.
×
