| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | IMB1; IPO1; IPOB; Impnb; NTF97 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human KPNB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KPNB1抗体的3篇示例文献(内容为模拟，建议通过学术数据库获取真实文献)：
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1. **文献名称**：*KPNB1 mediates NF-κB signaling and promotes glioblastoma progression*
**作者**：Wang, X. et al.
**摘要**：研究揭示了KPNB1通过调控NF-κB通路在胶质母细胞瘤中的作用，利用特异性抗体(如免疫组化)证实其高表达与肿瘤侵袭性正相关。
2. **文献名称**：*Antibody-based profiling of KPNB1 subcellular dynamics during viral infection*
**作者**：Garcia-Sastre, A. et al.
**摘要**：通过免疫荧光和Western blot分析KPNB1抗体，发现病毒感染导致KPNB1核定位改变，影响宿主细胞核转运机制。
3. **文献名称**：*Validation of KPNB1 antibodies for chromatin interaction studies*
**作者**：Kim, J. & Rosenfeld, M.G.
**摘要**：系统评估了多种KPNB1抗体的适用性，确定某克隆号抗体适用于ChIP-seq，揭示KPNB1与染色质重塑复合物的相互作用。
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**建议检索关键词**：
- "KPNB1 antibody validation"
- "Importin β1 cancer/therapeutic target"
- "KPNB1 nuclear transport assay"
可通过PubMed或Google Scholar查找最新文献，结合具体研究场景筛选。
**Background of KPNB1 Antibody**
KPNB1 (karyopherin β1), also known as importin β1. is a key nuclear transport receptor belonging to the importin β family. It plays a central role in mediating the transport of proteins from the cytoplasm into the nucleus through the nuclear pore complex (NPC). KPNB1 typically functions by binding to cargo proteins containing nuclear localization signals (NLS), either directly or via adaptor proteins like importin α, and facilitates their translocation across the NPC. This process is critical for regulating gene expression, cell cycle progression, and signal transduction.
Antibodies targeting KPNB1 are widely used in research to study nuclear-cytoplasmic transport mechanisms, protein localization, and interactions. They enable detection of KPNB1 expression levels, subcellular distribution (e.g., cytoplasmic vs. nuclear), and its association with disease pathologies. For instance, dysregulation of KPNB1 has been implicated in cancer (e.g., aberrant cell proliferation, drug resistance) and viral infections (e.g., hijacking nuclear transport by viral proteins). Additionally, KPNB1 antibodies are valuable tools in investigating neurodegenerative diseases linked to impaired nucleocytoplasmic trafficking.
These antibodies are commonly validated in applications such as Western blotting, immunofluorescence, immunoprecipitation, and immunohistochemistry, often with species cross-reactivity considerations. Their specificity and reliability make them essential for dissecting KPNB1's functional roles in both physiological and pathological contexts.
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