| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | M6PR |
| Uniprot No | P20645 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-185aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSTEEKTCDLVGEKGKESEKELALVKRLK PLFNKSFESTVGQGSDTYIYIFRVCREAGNHTSGAGLVQINKSNGKETVV GRLNETHIFNGSNWIMLIYKGGDEYDNHCGKEQRRAVVMISCNRHTLADN FNPVSEERGKVQDCFYLFEMDSSLACSPEISH |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of M6PR Recombinant Protein**
The **mannose-6-phosphate receptor (M6PR)**, also known as the cation-independent mannose-6-phosphate receptor (CI-M6PR), is a key transmembrane glycoprotein involved in lysosomal enzyme trafficking and activation. It binds to mannose-6-phosphate (M6P) residues on newly synthesized lysosomal enzymes in the Golgi apparatus, facilitating their transport to lysosomes. This receptor also plays roles in diverse physiological processes, including cell signaling, growth regulation, and tumor suppression.
**Recombinant M6PR protein** is engineered through genetic modification, typically expressed in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. The purified protein retains the functional domains necessary for M6P binding, enabling its use in studying lysosomal biogenesis, enzyme replacement therapies, and diseases linked to lysosomal dysfunction, such as mucolipidosis and certain neurodegenerative disorders.
Research applications include investigating receptor-ligand interactions, lysosomal storage disease mechanisms, and developing targeted drug delivery systems leveraging M6PR's ability to internalize extracellular ligands. Its recombinant form is also critical in structural studies to resolve M6PR’s architecture and dynamic interactions.
In summary, M6PR recombinant protein serves as a vital tool for both basic research and therapeutic development, bridging gaps in understanding lysosomal biology and related pathologies.
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