| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ALDM; ALDHI; ALDH-E2 |
| Entrez GeneID | 217 |
| clone | 4G6A3 |
| WB Predicted band size | 56.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于NEFM抗体的3篇参考文献示例(文献信息为模拟概括，仅供参考)：
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1. **文献名称**: *Neurofilament Medium (NEFM) as a Biomarker in Amyotrophic Lateral Sclerosis*
**作者**: Smith A, et al.
**摘要**: 本研究通过NEFM抗体检测ALS患者脑脊液和血清中的神经丝蛋白水平，发现NEFM浓度与疾病进展显著相关，提示其作为神经退行性病变的生物标志物潜力。
2. **文献名称**: *Immunohistochemical Localization of NEFM in Traumatic Brain Injury Models*
**作者**: Johnson R, Lee C.
**摘要**: 利用NEFM抗体对大鼠创伤性脑损伤模型进行免疫组化分析，揭示损伤后神经元轴突中NEFM的异常聚集，为轴突损伤机制提供形态学证据。
3. **文献名称**: *NEFM Antibody Cross-Reactivity in Peripheral Neuropathy Studies*
**作者**: Gupta S, et al.
**摘要**: 评估NEFM抗体在周围神经病变中的特异性，发现其与某些非靶蛋白的交叉反应性，强调抗体验证在神经病理学研究中的重要性。
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**注**：以上为示例文献，实际引用需查询具体数据库(如PubMed、Web of Science)获取真实研究。如需进一步协助定位某篇论文，可提供更详细的关键词。
**Background of NEFM Antibody**
Neurofilament medium (NEFM) is a subunit of neurofilaments (NFs), a class of type IV intermediate filaments critical for maintaining neuronal structure, axonal caliber, and intracellular transport. Neurofilaments consist of three subunits—light (NEFL), medium (NEFM), and heavy (NEFH)—which assemble into heteropolymers to form the neuronal cytoskeleton. NEFM, with a molecular weight of ~150 kDa, plays a key role in stabilizing axons and regulating signal transduction.
NEFM antibodies are immunological tools designed to detect and quantify NEFM proteins in research and diagnostics. These antibodies are widely used to study neuronal integrity, as neurofilament levels in cerebrospinal fluid (CSF) or blood correlate with axonal damage in neurodegenerative diseases (e.g., ALS, Alzheimer’s, Parkinson’s) and neurotrauma. Elevated NEFM expression or abnormal phosphorylation is linked to pathological aggregates observed in such disorders.
In research, NEFM antibodies enable visualization of neuronal morphology via immunohistochemistry (IHC) and Western blotting. They also aid in investigating neurofilament dynamics, including post-translational modifications (e.g., phosphorylation) that influence filament assembly and degradation. Commercially available NEFM antibodies are often validated for specificity across species (human, mouse, rat), supporting translational studies.
Overall, NEFM antibodies serve as vital biomarkers and experimental reagents in neuroscience, bridging molecular mechanisms of neurodegeneration to clinical diagnostics and therapeutic development.
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