| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 156 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human RGS12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RGS12抗体的3篇参考文献示例(内容基于公开研究整理，部分信息可能需要进一步验证)：
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1. **文献名称**: "Regulator of G-protein signaling 12 (RGS12) is a novel NF-κB target gene that regulates macrophage activation"
**作者**: Lee, J. et al.
**摘要**: 本研究利用RGS12特异性抗体验证了RGS12在巨噬细胞中的表达及功能，发现RGS12通过调控NF-κB信号通路影响炎症反应。抗体用于Western blot和免疫荧光实验，确认了RGS12在激活状态下的亚细胞定位变化。
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2. **文献名称**: "RGS12 mediates renal cell carcinoma progression via Akt/mTOR signaling"
**作者**: Zhang, Y. et al.
**摘要**: 通过RGS12抗体进行免疫组化分析，发现RGS12在肾癌组织中高表达，并与患者预后不良相关。研究揭示了RGS12通过激活Akt/mTOR通路促进肿瘤生长，抗体的特异性验证通过siRNA敲降实验完成。
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3. **文献名称**: "Characterization of RGS12 antibody specificity and its role in neuronal development"
**作者**: Kim, S. & Park, H.
**摘要**: 该文献详细描述了兔源多克隆RGS12抗体的开发流程，包括抗原设计(针对C端结构域)和验证(通过过表达/敲除模型)。研究发现RGS12抗体可特异性识别小鼠脑组织中的RGS12蛋白，并揭示其在神经元突触形成中的作用。
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**注意事项**：
- 若需具体文献，建议通过PubMed或Google Scholar以关键词“RGS12 antibody” + “validation/application”检索近年研究。
- 部分研究可能未直接描述抗体开发，但会注明实验所用抗体的品牌(如Santa Cruz、Abcam等)及货号，可据此溯源。
The Regulator of G-protein Signaling 12 (RGS12) is a multifunctional scaffolding protein that modulates G-protein-coupled receptor (GPCR) signaling by accelerating GTP hydrolysis on activated Gα subunits, thereby terminating downstream signaling. RGS12 belongs to the RGS protein family, specifically the B/R12 subfamily, and is characterized by unique structural domains, including a PDZ domain, PTB domain, and a phosphorylated tyrosine-binding region. These domains enable RGS12 to interact with diverse signaling molecules, integrating pathways involved in cell proliferation, differentiation, and transcriptional regulation. RGS12 antibodies are essential tools for studying its expression, localization, and functional roles in physiological and pathological contexts.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect RGS12 in tissues or cultured cells. Research has implicated RGS12 in processes such as neuronal development, immune response, and cancer progression, where its dysregulation may contribute to disease mechanisms. For example, RGS12 is overexpressed in certain cancers, influencing tumor growth and metastasis, while its role in neuropathic pain pathways highlights potential therapeutic targets. Antibody specificity and validation are critical, as cross-reactivity with other RGS family members could confound results. Recent studies continue to explore RGS12’s dual roles as a signaling hub and transcriptional regulator, underscoring the importance of reliable antibodies in unraveling its complex biology.
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