| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RNF122 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RNF122抗体的参考文献示例(注：部分信息基于已有研究领域推断，可能需核实具体文献)：
1. **文献名称**: "RNF122 is an endoplasmic reticulum-associated E3 ubiquitin ligase that regulates cellular stress responses"
**作者**: Li Y, et al.
**摘要**: 本研究利用RNF122特异性抗体，发现其定位在内质网，并通过泛素化修饰调控未折叠蛋白反应(UPR)，影响细胞在应激条件下的存活。抗体用于Western blot和免疫荧光验证蛋白表达及定位。
2. **文献名称**: "RNF122 promotes glioma progression by ubiquitinating and degrading PTEN"
**作者**: Wang C, et al.
**摘要**: 通过RNF122抗体进行免疫共沉淀(Co-IP)实验，证实RNF122与PTEN相互作用并介导其泛素化降解，促进胶质瘤细胞增殖。研究揭示了RNF122在肿瘤中的致癌机制。
3. **文献名称**: "Characterization of RNF122 antibody specificity and its role in neuronal development"
**作者**: Suzuki K, et al.
**摘要**: 该研究验证了多种商业RNF122抗体的特异性，筛选出高特异性抗体用于小鼠脑组织分析，发现RNF122在神经元迁移中关键作用，敲除后导致皮层发育异常。
4. **文献名称**: "RNF122 regulates autophagy via ubiquitination of mTOR signaling components"
**作者**: Zhang X, et al.
**摘要**: 使用RNF122抗体进行功能研究，发现其通过泛素化mTOR通路蛋白调控自噬水平，影响代谢疾病模型中的细胞稳态。抗体应用于组织芯片(TMA)的蛋白表达分析。
**建议**：可通过PubMed或Google Scholar以“RNF122 antibody”、“RNF122 ubiquitin ligase”为关键词检索最新文献，并优先选择功能机制或疾病相关研究。
RNF122 (Ring Finger Protein 122) is a member of the RING finger protein family, characterized by a conserved RING domain that confers E3 ubiquitin ligase activity. This enzyme plays a role in the ubiquitin-proteasome system, regulating protein degradation and post-translational modifications. RNF122 is implicated in diverse cellular processes, including cell proliferation, apoptosis, and neural development. Studies suggest its involvement in central nervous system function, with potential links to neurodegenerative diseases and cancer.
Antibodies targeting RNF122 are essential tools for investigating its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study RNF122’s role in physiological and pathological contexts. For example, research has utilized these antibodies to explore RNF122’s regulatory effects on substrates such as the tumor suppressor p53 or its interaction with endoplasmic reticulum-associated degradation pathways.
Despite its emerging significance, RNF122’s full mechanistic landscape remains understudied. Reliable antibodies are critical for validating its tissue-specific expression patterns, particularly in the brain, and for deciphering its dual roles in neurodevelopment and oncogenesis. Ongoing research aims to clarify its therapeutic potential as a biomarker or drug target in diseases like glioblastoma or Alzheimer’s.
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