| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | JM4 |
| WB Predicted band size | 19 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PRAF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRAF2抗体的3篇参考文献示例(注：以下内容为模拟示例，非真实文献)：
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1. **文献名称**: *PRAF2 modulates apoptosis signaling through interaction with Bcl-2 family proteins*
**作者**: Li X, Zhang Y, et al.
**摘要**: 本研究利用PRAF2特异性抗体，通过免疫共沉淀和Western blot技术，揭示了PRAF2与Bcl-2家族蛋白的相互作用机制，证明其在调控线粒体途径细胞凋亡中的作用，为癌症治疗靶点研究提供依据。
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2. **文献名称**: *Overexpression of PRAF2 in hepatocellular carcinoma and its clinical significance*
**作者**: Wang H, Chen J, et al.
**摘要**: 通过免疫组化(使用PRAF2多克隆抗体)分析肝癌组织样本，发现PRAF2在肝癌中高表达且与患者预后不良相关，提示其可能作为肝癌诊断的潜在生物标志物。
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3. **文献名称**: *Development and validation of a novel monoclonal antibody against human PRAF2 for flow cytometry applications*
**作者**: Smith KA, Brown R, et al.
**摘要**: 本文报道了一种针对人源PRAF2的新型单克隆抗体的开发与验证，该抗体在流式细胞术中表现出高特异性和灵敏度，成功应用于白血病细胞系的表面蛋白定量分析。
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如需真实文献，建议通过PubMed或Google Scholar以关键词“PRAF2 antibody”、“PRAF2 function”进行检索，并筛选近五年内与您研究领域相关的论文。
The PRAF2 (Prenylated Rab Acceptor 1 Family Member 2) protein, also known as RAPH1. is a member of the PRAF family involved in intracellular vesicular trafficking and membrane dynamics. It interacts with prenylated Rab GTPases, facilitating their proper subcellular localization and function in secretory pathways. PRAF2 is predominantly localized to the Golgi apparatus and endoplasmic reticulum, playing roles in maintaining organelle integrity and regulating protein transport. Studies suggest its involvement in apoptosis, where it may act as a pro-apoptotic factor by interacting with Bcl-2 family proteins or modulating mitochondrial pathways.
Aberrant PRAF2 expression has been linked to human diseases, particularly cancers. Overexpression is observed in certain tumors, correlating with poor prognosis, tumor progression, and metastasis, potentially through pathways affecting cell proliferation, migration, or chemoresistance. Conversely, reduced expression has been implicated in neurodevelopmental disorders, highlighting its tissue-specific roles.
PRAF2 antibodies are essential tools for detecting protein expression, localization, and interaction partners in research. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the exploration of PRAF2’s biological functions and pathological mechanisms. Commercial antibodies are typically generated using recombinant protein fragments or synthetic peptides, with validation across experimental models. Ongoing research focuses on clarifying PRAF2’s dual roles in cancer and neurological processes, positioning it as a potential diagnostic marker or therapeutic target.
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