| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NY-CO-1; SDCCAG1 |
| WB Predicted band size | 123 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NEMF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于NEMF抗体的3篇参考文献摘要(文献信息为虚构示例，实际文献需根据具体研究检索)：
1. **文献名称**：*NEMF蛋白在哺乳动物内质网相关降解中的功能研究*
**作者**：Smith A, et al.
**摘要**：本研究利用NEMF特异性抗体验证了NEMF蛋白在ERAD途径中的作用，发现其通过识别错误折叠蛋白并促进其泛素化降解，维持内质网稳态。
2. **文献名称**：*NEMF基因突变导致神经退行性疾病的分子机制*
**作者**：Chen L, et al.
**摘要**：通过免疫印迹(NEMF抗体)和免疫荧光技术，作者发现NEMF功能缺失会导致神经元内异常蛋白聚集，从而加速ALS小鼠模型的疾病进展。
3. **文献名称**：*NEMF与RNF5复合物的结构解析及其在质量控制中的作用*
**作者**：Wang Y, et al.
**摘要**：利用NEMF抗体进行免疫共沉淀实验，揭示了NEMF与E3泛素连接酶RNF5的相互作用，阐明了二者协同识别底物的结构基础。
---
**注**：实际文献需通过PubMed或Google Scholar以“NEMF antibody”或“NEMF function”为关键词检索。建议优先选择近5年内的研究。
NEMF (Nuclear Export Mediator Factor) antibodies are tools used to study the NEMF protein, a critical component in cellular protein quality control and RNA metabolism. NEMF, also known as RNF185 or C1orf103. is an evolutionarily conserved protein implicated in the ribosome-associated quality control (RQC) pathway. This pathway detects and degrades stalled translation products, preventing toxic protein aggregation. NEMF facilitates the ubiquitination of incomplete polypeptides via its interaction with the E3 ligase LTN1. marking them for proteasomal degradation.
Mutations in NEMF have been linked to neurological disorders, including childhood-onset neurodegeneration and motor neuron diseases, highlighting its role in neuronal health. Additionally, NEMF participates in regulating mRNA stability and translation under stress conditions.
NEMF antibodies are essential for detecting protein expression, localization, and interaction partners in experimental models (e.g., Western blot, immunofluorescence). They enable research into disease mechanisms, such as how NEMF dysfunction contributes to protein aggregation in neurodegeneration. Recent studies also explore its potential role in cancer biology, where altered protein quality control pathways influence tumor progression. These antibodies thus serve as vital reagents for unraveling NEMF's multifaceted roles in cellular homeostasis and pathology.
×
