| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SIN3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SIN3B抗体的3篇假设性参考文献示例(仅供参考，具体文献需通过学术数据库验证)：
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1. **文献名称**：*SIN3B regulates cell cycle exit and differentiation via histone deacetylase activity*
**作者**：Davidson, K.C., et al.
**摘要**：本研究利用SIN3B抗体通过染色质免疫沉淀(ChIP)和Western blot技术，证明SIN3B通过招募HDAC复合物至特定细胞周期基因启动子区，促进干细胞向神经谱系分化时的细胞周期退出。
2. **文献名称**：*Transcriptional repression by SIN3B-HDAC complex in cancer metabolism*
**作者**：Grandinetti, K.B., et al.
**摘要**：通过免疫共沉淀(Co-IP)和SIN3B抗体的敲除实验，研究发现SIN3B与HDAC1/2结合，抑制糖酵解相关基因表达，在肺癌细胞中调控代谢重编程，影响肿瘤生长。
3. **文献名称**：*SIN3B interacts with p53 to modulate DNA damage response*
**作者**：Bansal, N., et al.
**摘要**：采用SIN3B特异性抗体的功能研究显示，SIN3B通过结合p53并调控其靶基因的染色质状态，参与DNA损伤修复过程，缺失SIN3B会导致基因组不稳定性和细胞凋亡抵抗。
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**注**：以上为基于SIN3B已知功能的假设性示例，实际文献需查阅PubMed、Web of Science等平台，结合关键词“SIN3B antibody”或“SIN3B function”筛选近年研究。
The SIN3B antibody is a crucial tool in studying the SIN3B protein, a key component of the SIN3 transcriptional regulatory complex. SIN3B, along with its paralog SIN3A, functions as a scaffold protein in histone deacetylase (HDAC)-dependent chromatin remodeling complexes, primarily interacting with HDAC1 and HDAC2. These complexes repress gene expression by deacetylating histones, leading to chromatin condensation and transcriptional silencing. SIN3B plays roles in cell cycle regulation, differentiation, and apoptosis, with dysregulation linked to cancers and developmental disorders.
SIN3B antibodies are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence to detect SIN3B expression levels, protein-protein interactions, and subcellular localization. Given SIN3B's nuclear localization, these antibodies help map its involvement in gene regulatory networks. Many commercially available SIN3B antibodies are raised against specific epitopes, such as N-terminal or C-terminal regions, and are validated in human, mouse, or rat samples. Specificity is often confirmed using siRNA knockdown or SIN3B-deficient cell lines. Researchers rely on these antibodies to explore SIN3B's context-dependent roles, including its cooperation or competition with SIN3A in various tissues and disease models. Proper validation remains critical due to potential cross-reactivity with SIN3A or other paralogs in certain experimental conditions.
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