| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAGED1 |
| Uniprot No | Q9Y5V3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 504-760aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML KDYTKVPIKR SEMLRDIIRE YTDVYPEIIE RACFVLEKKF GIQLKEIDKE EHLYILISTP ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLR KLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA QFMEAADEAL DALDAAAAEA EARAEARTRM GIGDEAVSGP |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAGED1 Recombinant Protein**
MAGED1 (Melanoma-associated antigen D1), also known as NRAGE, is a member of the MAGE protein family, which shares homology with cancer-testis antigens. Unlike many MAGE proteins, MAGED1 is ubiquitously expressed in normal tissues, particularly in the nervous system, and plays diverse roles in cellular processes such as apoptosis, differentiation, and signaling. Structurally, MAGED1 contains a conserved MAGE homology domain (MHD) involved in protein-protein interactions and a unique C-terminal region that mediates binding to specific partners.
Research highlights its involvement in key pathways, including interactions with p75 neurotrophin receptor (p75NTR) to regulate neuronal apoptosis and differentiation, as well as modulation of Wnt/β-catenin signaling. MAGED1 also interacts with hypoxia-inducible factor 1α (HIF-1α), linking it to cellular responses under low-oxygen conditions. Dysregulation of MAGED1 has been implicated in neurodevelopmental disorders, metabolic syndromes, and cancers (e.g., melanoma, neuroblastoma), where it may act as a tumor suppressor or promoter depending on context.
Recombinant MAGED1 protein is engineered for in vitro studies to dissect its molecular functions, structural features, and interactions. Produced via bacterial or mammalian expression systems, it enables biochemical assays (e.g., pull-down, ELISA), cell-based studies, and therapeutic target validation. Its applications span neuroscience, oncology, and metabolic disease research, with potential implications for developing diagnostic or therapeutic strategies.
In summary, MAGED1 recombinant protein serves as a critical tool for unraveling the multifunctional roles of MAGED1 in health and disease, offering insights into its therapeutic targeting.
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